安石榴苷对绵羊精液室温保存效果影响的研究

Effect of punicalagin on the quality of sheep semen stored at room temperature

为研究安石榴苷对绵羊精液常温保存效果的影响,在以“Tris+葡萄糖+果糖”为主的绵羊精液基础稀释液中添加安石榴苷,分别配制成含不同浓度(0,10.0,12.5,15.0,17.5,20.0μmol/L)安石榴苷的稀释液后,常温条件下稀释、保存多浪羊精液,通过检测精子的活率、活力、顶体完整性、质膜完整性,以及精液中具有抗氧化性的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,综合评价精液常温保存的效果。结果表明:15.0μmol/组精液保存72 h后,精子的活率(55.95%)、活力(42.82%)、顶体完整性(48.61%)、质膜完整性(44.40%)、CAT活性(4.61 U/mL)和SOD活性(62.70 U/mg)最高,且均显著高于对照组(P<0.05)。说明以“Tris+葡萄糖+果糖”为主的多浪羊精液基础稀释液中添加15.0μmol/L安石榴苷,能提高多浪羊常温保存效果。

In order to study the effect of punicalagin on the sheep semen quality,semen samples of Duolang sheep were stored at room temperature after being diluted in Tris-glucose-fructose-based extender with different concentrations(0,10.0,12.5,15.0,17.5,20.0μmol/L)of punicalagin,and used for evaluating the sperm viability,motility,acrosome integrity,plasma membrane integrity,and antioxidant superoxide dismutase(SOD)activity and catalase(CAT)content in the semen after 24,48 and 72 h,respectively.The results showed that at room temperature,The sperm motility(55.95%),motility(42.82%),acrosome integrity(48.61%),plasma membrane integrity(44.40%),CAT activity(4.61 U/mL)and SOD activity(62.70 U/mg)were the highest in the semen samples diluted with extender containing 15.0μmol/L punicalagin after 72 hours,which were significantly higher than those in the control group(P<0.05).In conclusion,the quality of Duolang sheep semen stored at room temperature could be improved when diluted with Tris-glucose-fructose-based extender containing 15.0μmol/L punicalagin.