二套小麦-簇毛麦染色体附加系苗期耐盐性及籽粒硒和叶酸的含量

Salt Tolerance at Seedling Stage and Analysis of Selenium and Folic Acid Content in Seeds in Two Sets of Wheat-Dasypyrum villosum Chromosom Additional Lines

簇毛麦(Dasypyrum villosum)作为小麦的近缘种,具有许多优良基因,是改善小麦耐盐性和提高小麦营养品质的理想材料。以小麦-簇毛麦#2和小麦-簇毛麦#3二体附加系为材料,进行耐盐性鉴定及籽粒中硒和叶酸含量的测定。发现小麦-簇毛麦#2附加系和#3附加系在盐胁迫下的萌发率分别为16.67%-43.33%、26.67%-70.00%,均高于对照中国春(6.65%);盐胁迫下附加系DA3V#3、DA7V#3、DA2V#3和DA5V#2的萌发率分别为70.00%、56.67%、53.33%和43.33%;盐胁迫下附加系的生长正常,株高和根长均大于对照中国春,其中DA4V#2和DA2V#3的株高分别达到15.2和16.1 cm,DA2V#3根长为3.4 cm;在14个附加系和对照中,DA2V#2和DA2V#3籽粒硒含量最高,分别为8.47和7.60μg/g。小麦-簇毛麦#2附加系和#3附加系籽粒中叶酸含量为9.00-26.10μg/100 g,大多数附加系高于对照中国春(10.98μg/100 g),其中,附加系DA4V#2和DA6V#2与对照差异达极显著水平,DA6V#2比对照增加2.4倍。簇毛麦2V#3、3V#3和5V#2染色体可能存在耐盐相关基因,2V染色体可能存在富硒相关基因,6V#2染色体可能携带籽粒叶酸合成的相关基因。

Dasypyrum villosum as a related species of wheat has many excellent genes,and it is an ideal material to improve the salt tolerance and nutritional quality of wheat.In this study,wheat-D.villosum#2 and wheat-D.villosum#3 disomic addition lines were applied for the identification of salt tolerance and determination of selenium and folic acid contents in seeds.It was found that the germination rates of the two sets of additional lines under salt stress were 16.67%-43.33%and 26.67%-70.00%,respectively,which were higher than the control Chinese Spring(CS)(6.65%).The germination rates of the addition lines DA3V#3,DA7V#3,DA2V#3 and DA5V#2 under salt stress were 70.00%,56.67%,53.33%and 43.33%,respectively.The additional lines grew normally under salt stress,and the plant height and root length of the additional lines under salt stress were higher than those of the control CS,among which the plant height of DA4V#2 and DA2V#3 reached cm and 16.1 cm,respectively,and the root length of DA2V#3 was 3.4 cm.The highest grain selenium content was tested in the addition line DA2V#2(8.47μg/g)and DA2V#3(7.60μg/g)containing chromosome 2V in the 14 addition lines.The folic acid content in the seeds of wheat-D.villosum additional lines#2 and#3 was 9.00-26.10μg/100 g,among which most of the addition lines folic acid content were higher than the control(10.98μg/100 g).The difference between DA4V#2 or DA6V#2 and the CS was extremely significant in folic acid content,and the folic acid content in DA 6V#2 was 2.4-fold higher than that in CS.The results inferred that there might be salt tolerance related genes on chromosomes 2V#3,3V#3,and 5V#2,grain selenium enrichment related genes on chromosome 2V,and grain folic acid biosynthesis related genes on chromosome 6V#2.