金钱草及广金钱草对大鼠肾草酸钙结石相关代谢产物作用的研究

Effects of christina loosestrife and snowbellleaf tickclover herb on kidney calcium oxalate calculi related metabolites in rats

目的对比金钱草与广金钱草抑制大鼠肾草酸钙结石的具体机制与作用效果。方法54只SPF级SD雄性大鼠适应性喂养1周至体重180~200 g,使用乙二醇灌胃法建立SD大鼠肾草酸钙结石模型,而后将大鼠按照随机数字表法分为9组处理并进行对照,分别为健康对照组(A组),阳性对照组(建模组,B组),金钱草低、中、高剂量组(C1、C2、C3组,共3组),广金钱草低、中、高剂量组(D1、D2、D3组,共3组),疗效对照组(枸橼酸氢钾钠组,E组),每组各6只。4周后收集标本测定各组大鼠血尿生化指标,并行Von Kossa染色检测肾草酸钙晶体,在偏振光显微镜下观察大鼠肾组织草酸钙结晶沉积情况,利用测算照片中阳性面积百分比与相关血尿生化指标判断两者药效差异。计量资料以均数±标准差(x±s)表示,组间比较采用单因素方差分析,两组间比较采用SNK-q检验;结晶形成情况组间比较采用Kruskal-Wallis检验。结果与阳性对照组相比,高剂量的广金钱草相对金钱草能显著降低肾草酸钙结石大鼠血肌酐水平,应用金钱草后血肌酐水平为(86.70±11.49)μmol/L,应用广金钱草后血肌酐水平为(70.72±9.08)μmol/L,两者差异有统计学意义(P<0.01);高剂量金钱草、广金钱草均能显著升高尿镁水平,降低血尿素水平,两者相比差异无统计学意义(P>0.05);与阳性对照组相比,给予高剂量金钱草(P<0.0001)与高剂量广金钱草(P<0.0001)均能显著抑制大鼠肾草酸钙晶体形成,保护大鼠肾脏,两者作用效果相比差异无统计学意义(P>0.05);给予金钱草与广金钱草均未能观察到显著升高尿pH值与显著降低尿钙、尿草酸、24 h尿量、血钙、血磷、血镁、血尿酸和肾草酸含量的效果。结论广金钱草抑制大鼠肾草酸钙结石发生的功效优于金钱草,对肾功能具有更好的保护作用。

Objective To compare the specific mechanism and effects between christina loosestrife and snowbellleaf tickclover herb on kidney calcium oxalate calculi in rats.Methods A total of 54 SPF grade SD male rats were fed adaptically for 1 week to 180-200 g,the models of rats with kidney calcium oxalate calculi were established by intragastric administration with glycol,and divided into nine groups according to random number table method and controlled,which were healthy control group(group A),positive control group(model group,group B),low,medium and high doses of christina loosestrife groups(C1,C2,C3,3 groups),low,medium and high doses of snowbellleaf tickclover herb groups(D1,D2,D3,3 groups),therapeutic control group(potassium sodium hydrogen citrate group,group E),6 rats in each group.After 4 weeks,samples were collected to determine the urine and serum biochemical indexes of each group,and Von Kossa staining was used to detect kidney calcium oxalate crystals.Calcium oxalate crystal deposition in kidney tissues of rats was observed under polarization microscope,and the difference of efficacy between the two drug effects was determined by the percentage of positive area in photos and the urine and serum biochemical indexes.The measurement data were expressed as mean±standard deviation(x±s),one-way analysis of variance was used for comparison between groups,and SNK-q test was used for comparison between two groups.Kruskal-Wallis test was used to compare crystal formation between groups.Results Compared with the positive control group and christina loosestrife groups,high dose of snowbellleaf tickclover herb could significantly reduce serum creatinine level(P<0.01),the mean serum creatinine of rats with christina loosestrife was(86.70±11.49)μmol/L,that of rats with snowbellleaf tickclover herb was(70.72±9.08)μmol/L,the difference was statistically significant(P<0.01).High dose of christina loosestrife and snowbellleaf tickclover herb could significantly increase urinary magnesium and decrease serum urea levels,and there was no statistical significance between them(P>0.05).Compared with the positive control group,high dose of christina loosestrife(P<0.0001)and snowbellleaf tickclover herb(P<0.0001)could both inhibit the formation of calcium oxalate crystals and protect the kidney of rats,and there was no statistical significance between the two effects(P>0.05).The levels of urine pH value could not be increased,while the levels of urinary calcium urinary oxalic acid and 24 h urine volume,serum calcium,serum phosphorus,serum magnesium,blood uric acid and content of kidney oxalate could not be decreased significantly after using these two drugs.Conclusion Snowbellleaf tickclover herb is better than christina loosestrife in preventing recurrence of kidney calcium oxalate calculi and protecting renal function.