水中大环内酯类、磺胺类和喹诺酮类抗生素前处理分析方法优化

Optimization on Pretreatment Methods of Macrolides, Sulfonamides and Quinolones Antibiotics in Water

本文研究在不同pH和EDTA加入量下对水中大环内酯类、磺胺类和喹诺酮类抗生素目标物进行固相萃取回收率的影响,探索这三类抗生素分析的最优前处理条件。结果表明,8种大环内酯类抗生素、16种磺胺类抗生素在pH=4.0条件下和9种喹诺酮类抗生素在pH=12.0条件下分别可以获得较好的回收率,EDTA的加入对加标回收率有显著作用。抗生素的准确分析,有效的前处理技术是基础,对不同种类的抗生素进行前处理技术的探索和优化,对未来抗生素检测技术的提升有着重要意义。

To explore optimum pretreatment condition of macrolides,sulfonamides and quinolones antibiotics analysis by the recovery effect of the SPE methods for these three types of antibiotics in water,and through different pH value and the addition of EDTA.The result implies that 8 types of macrolides and 16 types of sulfoamides,or 9 types of quinolones antibiotics can gain better recovery rate under the condition of pH=4 and pH=12,respectively.It is significant that the addition of EDTA has positive on recovery rate.The accurate analysis of the antibiotics is based on the effective pretreatment techniques.It is positive to promote the detection technology in the future by the exploration and optimization of various antibiotics on pretreatment methods.