多杀性巴氏杆菌快速检测方法研究进展

Research Progress of Rapid Detection Methods for Pasteurella Multocida

多杀性巴氏杆菌(P.multocida)是一种可引起畜禽巴氏杆菌病的病原菌,其感染主要引起动物出血性败血症或传染性肺炎,给我国畜禽养殖业造成巨大的经济损失。快速、准确检测P.multocida对于畜禽巴氏杆菌病的科学防控具有重要意义。该文综述近年国内外报道的P.multocida快速检测方法,包括免疫学检测技术(酶联免疫吸附试验、间接血凝试验、免疫层析技术、免疫斑点试验、基于免疫磁珠的显色法等)和核酸检测技术(PCR及其衍生技术、多种等温扩增技术、荧光原位杂交以及基于核酸的新型检测方法等),以期为我国科学防控畜禽巴氏杆菌病提供参考方法。

Pasteurella multocida(P.multocida)is a pathogenic bacterium that can cause pasteurellosis in livestock and poultry.Its infection mainly causes hemorrhagic septicemia or infectious pneumonia in animals,which has caused huge economic losses to our farming industry.Rapid and accurate detection of P.multocida is of great significance for the scientific prevention and control of animal pasteurellosis.The paper reviewed the rapid detection methods for P.multocida reported in recent years,including immunology-based detection methods(enzyme-linked immunosorbent assay,Indirect hemagglutination assay,immunochromatographic techniques,dot immunobinding assay,immunomagnetic bead-based colorimetric method,etc.)and nucleic acid-based detection methods(PCR and its derivative techniques,multiple isothermal amplification techniques,fluorescent in situ hybridization,and novel nucleic acid-based detection methods,etc.),with a view to providing methodological references for the scientific prevention and control of livestock and poultry pasteurellosis in China.