肝纤维化相关长链非编码RNA1在慢性乙型肝炎肝硬化患者的表达及临床意义

Expression and clinical significance of lnc-LFAR1 in patients with chronic hepatitis B cirrhosis

目的 探讨慢性乙型病毒性肝炎(chronic hepatitis B, CHB)肝硬化患者血清肝纤维化相关lncRNA1(lncLFAR1)表达水平及临床意义,旨在分析其与白介素-6(interleukin-6, IL-6)、白介素-1β(IL-1β)及肝功能的相关性。方法 选取2016年3月-2019年12月东莞市人民医院诊治的80例CHB肝硬化患者、80例CHB患者分别为肝硬化组、CHB组,并选取同期80例体检健康者为健康组。分析CHB肝硬化患者血清lnc-LFAR1、IL-6、IL-1β水平与肝功能指标白蛋白(albumin, ALB)、谷丙转氨酶(alanine aminotransferase, ALT),及血清lnc-LFAR1表达水平与IL-6、IL-1β的相关性;比较不同Child-Pugh分级CHB肝硬化患者血清lnc-LFAR1、IL-6、IL-1β、ALB、ALT水平。结果 肝硬化组患者血清lnc-LFAR1、IL-6、IL-1β、ALT水平[(1.85±0.62)、(41.76±13.92)ng/mL、(7.78±1.95)pg/mL、(148.37±29.67)U/L]高于CHB组[(1.42±0.47)、(23.56±7.85) ng/mL、(5.42±1.41) pg/mL、(87.59±17.52) U/L]、健康组[(1.01±0.34)、(6.70±2.23)ng/mL、(3.13±0.78)pg/mL、(15.44±3.10)U/L](P<0.05),ALB水平(30.54±3.82)g/L低于CHB组(37.27±4.34)g/L、健康组(45.26±5.66)g/L(P<0.05);CHB组患者血清lnc-LFAR1、IL-6、IL-1β、ALT水平高于健康组(P<0.05),ALB水平低于健康组(P<0.05);CHB肝硬化患者血清lnc-LFAR1、IL-6、IL-1β水平与ALB均呈负相关(P<0.05),与ALT均呈正相关(P<0.05);CHB肝硬化患者血清lnc-LFAR1表达水平与IL-6、IL-1β呈正相关(r=0.598、0.571,P<0.05);随Child-Pugh分级升高,CHB肝硬化患者血清lnc-LFAR1、IL-6、IL-1β、ALT水平逐渐升高(P<0.05),ALB水平逐渐降低(P<0.05)。结论 CHB肝硬化患者血清lnc-LFAR1表达水平较高,与IL-6、IL-1β及ALB、ALT有关,检测血清lncLFAR1表达水平有助于临床评估CHB肝硬化患者病情。

Objective To investigate the expression level and clinical significance of serum liver fibrosis-associated lncRNA1(lnc-LFAR1)in patients with chronic hepatitis B cirrhosis,aiming to analyze its correlation with interleukin-6(IL6),interleukin-1β(IL-1β),and liver function.Methods Patients with chronic hepatitis B(CHB)cirrhosis and CHB diagnosed and treated in Dongguan City People's Hospital from March 2016 to December 2019 were selected and divided into the liver cirrhosis group(n=80)and the CHB group(n=80),and 80 healthy people with physical examination during the same period were selected as healthy group.The serum levels of lnc-LFAR1,interleukin-6(IL-6),albumin(ALB),interleukin-1β(IL-1β)and liver function indicators,including albumin(ALB)and alanine aminotransferase(ALT)were measured and analyzed.The correlation between serum lnc-LFAR1 expression level and IL-6,IL-1βwas assessed,and the levels of lncLFAR1,IL-6,IL-1β,ALB and ALT were compared among patients with CHB cirrhosis of different Child-Pugh grades.Results The serum levels of lnc-LFAR1,IL-6,IL-1β and ALT in the patients with liver cirrhosis[(1.85±0.62),(41.76±13.92)ng/mL,(7.78±1.95)pg/mL,(148.37±29.67)U/L]were higher than those in the CHB group[(1.42±0.47),(23.56±7.85)ng/mL,(5.42±1.41)pg/mL,(87.59±17.52)U/L]and the healthy group[(1.01±0.34),(6.70±2.23)ng/mL,(3.13±0.78)pg/mL,(15.44±3.10)U/L](P<0.05),while the ALB levels(30.54±3.82)g/L were lower than those in the CHB group(37.27±4.34)g/L and the healthy group(45.26±5.66)g/L(P<0.05).Serum lnc-LFAR1,IL-6,IL-1β and ALT levels in the CHB group were higher than those in the healthy group(P<0.05),and ALB levels were lower than those in the healthy group(P<0.05);the serum levels of lnc-LFAR1,IL-6,IL-1β in patients with CHB cirrhosis were negatively correlated with ALB(P<0.05),and positively correlated with ALT(P<0.05);the serum expression level of lnc-LFAR1 in patients with CHB cirrhosis was positively correlated with IL-6 and IL-1β(r=0.598,0.571,P<0.05);with the increase of Child-Pugh grade,the serum levels of lnc-LFAR1,IL-6,IL-1β,and ALT in patients with CHB cirrhosis gradually increased(P<0.05),and the level of ALB gradually decreased(P<0.05).Conclusions Serum lnc-LFAR1 expression level is higher in patients with CHB cirrhosis,which is obviously related to IL-6,IL-1β,ALB and ALT.Therefore,the evaluation of serum lnc-LFAR1 expression level is helpful in the clinical assessment of the condition of CHB cirrhosis patients.