通过SP/NK-1R信号通路探讨参榆洗液对痔术后大鼠的止痛机制

Mechanism of Shenyu lotion relieving pain in rats after hemorrhoid surgery by regulating the SP/NK-1R signaling pathway

目的:探究参榆洗液通过调节SP/NK-1R信号通路对痔术后大鼠疼痛的改善作用。方法:将40只SD大鼠随机分为参榆洗液组(实验组)、金玄痔科熏洗散组(对照组)、模型组、空白组,每组各10只,完成模拟外痔切除术后,各组予以相应药物治疗及换药刺激,连续7 d,期间观察大鼠疼痛相关动物学行为。分别于第4天、第7天采集数据后麻醉处死各组半数大鼠,取肛周组织行HE染色观察以及检测肛周组织中P物质及NK-1受体的蛋白含量。结果:与空白组比较,模型组大鼠组织水肿与炎性浸润明显(P<0.05);与模型组比较,实验组、对照组可减少痔术后大鼠疼痛导致的扭体反应次数,延长首次舔舐肛周创面的潜伏时间,缩短舔舐肛周总时间,减少肛周创面组织中P物质、NK-1受体的阳性表达(均P<0.05)。与对照组比较,实验组对以上各项指标的改善效果更优(均P<0.05)。结论:参榆洗液可通过下调肛周组织P物质和NK-1受体表达抑制SP/NK-1R通路,阻碍疼痛信号的产生和传递,减轻组织水肿和炎性浸润,缓解大鼠的疼痛反应,故抑制SP/NK-1R通路是参榆洗液缓解痔术后疼痛的机制之一。

Objective:To investigate the effect of Shenyu lotion in improving pain in rats after hemorrhoid surgery by regulating the SP/NK-1R signaling pathway.Methods:A total of 40 Sprague-Dawley rats were randomly divided into Shenyu lotion group(experimental group),Jinxuan Zhike fumigation-washing powder group(control group),model group,and blank group,with 10 rats in each group.After the simulation of external hemorrhoidecto-my,each group was given corresponding pharmacotherapy and dressing change for 7 consecutive days,and pain-re-lated behaviors were observed for each group.Half of the rats in each group were sacrificed on days 4 and 7 after data collection.Perianal tissue was collected for HE staining,and the protein content of substance P and NK-1 re-ceptor in perianal tissue was measured.Results:Compared with the blank group,the model group had marked tissue edema and inflammatory infiltration(P<0.05),and compared with the model group,the experimental group and the control group had a significant reduction in the number of writhing response due to pain,a significantly longer latency time to first licking of perianal wound,a significantly shorter total time of licking of perianal region,and significant reductions in the positive expression of substance P and NK-1 receptor in perianal wound(all P<0.05).Compared with the control group,the experimental group had significantly better improvements in the above indicators(all P<0.05).Conclusion:Shenyu lotion can inhibit the SP/NK-1R pathway by downregulating the expression of sub-stance P and NK-1 receptor in perianal tissue,block the generation and transmission of pain signal,alleviate tissue edema and inflammatory infiltration,and relieve pain response,and therefore,inhibition of the SP/NK-1R pathway might be one of the mechanisms for Shenyu lotion in relieving pain after hemorrhoid surgery.