基于荧光动力学的海洋初级生产力快速原位传感技术

Research on Fast in Situ Sensing Technology for Marine Gross Primary Productivity Based on Fluorescence Dynamics Method

海洋初级生产力(GPP)是海洋生态系统物质循环和能量流动的基础环节,是评价海洋生态环境状况的重要指标之一。传统GPP测量方法操作过程繁琐、测量周期长且时效性差。文中围绕海洋GPP快速监测需求,以叶绿素荧光作为浮游植物光合作用过程探针,研究了多波段可变光脉冲诱导荧光动力学技术,研发了海洋GPP快速原位传感器,并在北极、黄渤海和南海等海域开展了海试应用,获取了我国黄海近海、渤海湾、南海岛礁周边,以及太平洋及北冰洋部分海域表层海水的GPP空间分布情况,为海洋生态环境监测和科学研究提供了大量实时观测数据。海试结果表明,基于多波段可变光脉冲诱导荧光动力学技术能够快速准确地获取GPP,可作为海洋生态环境调查和海洋碳汇评估的先进技术手段。

Marine gross primary productivity(GPP)constitutes a fundamental element of the biological matter cycle and energy flow within the marine ecosystem,serving as a key indicator for assessing the sate of marine ecological environment.Traditional methods of measuring GPP are often laborious,with long measurement period and poor immediacy.To address the pressing need for expeditious monitoring of marine GPP in ecological surveys and carbon sink assessment,we explored multi-wavelength variable light pulse-induced fluorescence kinetic technology.This approach leverages chlorophyll fluorescence as a probe of phytoplankton photosynthetic activity and culminated in the development of an in-situ sensor.Subsequently,we applied this sensor in trials across the Arctic,Yellow Sea,Bohai Sea,and South China Sea.The resulting spatial distribution of GPP across these regions has yielded extensive real-time observational data,contributing substantially to the monitoring and scientific research of the marine ecological environment.Sea trials outcomes demonstrate that the multi-band variable light pulse-induced fluorescence kinetic technology can rapidly and accurately estimate GPP,offering notable advantages such as speed,accuracy,stability,and reliability in measurement.This ultimately provides an advanced technical resource for marine ecological investigations and carbon sink assessments.