响应面法改良牟氏角毛藻培养基

Optimization of Culture Medium of Chaetoceros muelleri by Response Surface Methodology

为优化牟氏角毛藻的培养基配方,提高牟氏角毛藻生长速度及细胞密度,采用响应面法筛选f/2培养基中的NaNO_(3)、NaH_(2)PO_(4)·H2O、Na_(2)SiO_(3)和C6H8FeNO7质量浓度并进行验证。结果表明,根据单因素试验筛选出牟氏角毛藻相对适宜的营养盐质量浓度范围为:NaNO_(3)10~30 mg·L^(-1),NaH_(2)PO_(4)·H2O 1.0~3.0 mg·L^(-1),Na_(2)SiO_(3)20~80 mg·L^(-1),C6H8FeNO70.1~0.5 mg·L^(-1);通过响应面优化,4种营养盐对牟氏角毛藻生长的影响强弱依次为NaNO_(3)>Na_(2)SiO_(3)>NaH_(2)PO_(4)·H2O>C6H8FeNO7,最佳质量浓度分别为NaNO_(3)21.8 mg·L^(-1)、NaH_(2)PO_(4)·H2O 2.93 mg·L^(-1)、Na_(2)SiO_(3)54.2 mg·L^(-1)、C6H8FeNO70.158 mg·L^(-1)。使用优化后的培养基培养该藻,密度提高27.95%,显著高于f/2培养基组的细胞密度(P<0.05),第4天后试验组牟氏角毛藻光合系统PSⅡ最大光能转换效率显著高于对照组(P<0.05)。综上所述,该方法筛选得出的营养盐质量浓度可以显著提高牟氏角毛藻的生长速度及细胞密度,结果为牟氏角毛藻的高密度培养提供数据参考。

In order to optimize the medium formula of Chaetoceros muelleri and improve its growth rate and cell density,response surface methodology was used to screen and verify the mass concentration of NaNO_(3),NaH_(2)PO_(4)·H_(2)O,Na_(2)SiO_(3)and C6H8FeNO7 in f/2 medium.The results showed that,in accordance with single factor,the nutrient concentrations suitable for C.muelleri were screened,and the mass concentrations of NaNO_(3),NaH_(2)PO_(4)·H_(2)O,Na_(2)SiO_(3)and C6H8FeNO7 were 10~30,1.0~3.0,20~80,0.1~0.5 mg·L^(-1),respectively.After being optimized by responding surface,the effects of four nutrient salts on the growth of C.muelleri were as follows:NaNO_(3)>Na_(2)SiO_(3)>NaH_(2)PO_(4)·H_(2)O>C6H8FeNO7,and the optimal mass concentration of them were:NaNO_(3)21.8 mg·L^(-1),NaH_(2)PO_(4)·H_(2)O 2.93 mg·L^(-1),Na_(2)SiO_(3)54.2 mg·L^(-1)and C6H8FeNO70.158 mg·L^(-1).Cell density of C.muelleri in optimized culture medium group enhanced 27.95%,significantly higher than that of f/2 medium group(P<0.05).At 4th days,the maximum light conversion efficiency of photosynthetic system PSII in experimental group was significantly higher than that in control group(P<0.05).To sum up,the selected nutrient mass concentration could significantly improve the growth rate and cell density of C.muelleri,which could provide data reference for high-density culture of C.muelleri.