橙皮苷对DOCA/Salt高血压大鼠心肾组织损害的保护作用

The protective effect of hesperidin on cardiac and renal tissue damage in DOCA/Salt hypertensive rats

目的探讨橙皮苷(hesperidin,HES)对高血压大鼠心肾损害的保护作用及可能机制。方法雄性SD大鼠18只随机分为3组:对照组(Ctrl)、高血压模型组(DOCA/Salt)、橙皮苷给药组(DOCA/Salt+HES)。HES持续给药4周,检测血压、血清肌酐、尿素氮等指标,HE、马松和天狼星红染色观察心、肾组织病理改变,Western blot检测α-SMA、collagenⅠ和TGF-β等蛋白表达,qRT-PCR分别检测Nlrp3、TNF-α、IL-1β、IL-6和NOXs的mRNA表达。结果与模型组比较,HES给药明显减缓DOCA/Salt高血压的发生,改善高血压大鼠肾功能指标,减少肾脏和心脏组织纤维化,降低α-SMA、collagenⅠ和TGF-β的表达,抑制Nlrp3,TNF-α、IL-1β和IL-6等炎症因子释放,减少肾脏和心脏组织中NOXs表达。结论HES具有延缓高血压的发生,改善高血压肾脏和心脏组织损害的保护作用,其作用机制可能与HES减少炎症反应和氧化应激有关。

Aim To investigate the protective effect of hesperidin(HES)on cardiorenal damage induced by DOCA/Salt hypertension and the underlying mechanisms.Methods Eighteen male SD rats were randomly divided into normal group(Ctrl),model group(DOCA/Salt),and DOCA/Salt with hesperidin group(DOCA/Salt+HES).HES was administered for four weeks.Blood pressure,serum creatinine and blood urea nitrogen were measured.The pathological changes in heart and kidney were examined by HE,Masson and Sirius red staining.The expression ofα-SMA,collagenⅠand TGF-βwere detected by Western blot.The mRNA levels of Nlrp3,TNF-α,IL-1β,IL-6 and NOXs were measured using qRT-PCR.Results Compared with the model group,HES administration significantly attenuated the occurrence of DOCA/Salt hypertension,improved renal function indicators of hypertensive rats,reduced renal and cardiac fibrosis,deduced the expression ofα-SMA,collagenⅠand TGF-β,inhibited the expression of Nlrp3,TNF-α,IL-1βand IL-6,and decreased the expression of NOXs in renal and cardiac tissues.Conclusions HES can delay the occurrence of hypertension and protect against hypertension-induced renal and cardiac tissue damage,which may be related to the reduction of inflammatory reaction and oxidative stress by HES.