烙灸介导Wnt信号通路对脊髓损伤大鼠内源性神经干细胞增殖分化影响

Effect of LaoMoxibustion Mediated Wnt Signal Pathway on Proliferation and Differentiation of Endogenous Neural Stem Cells in Rats with Spinal Cord Injury

目的:探究烙灸介导Wnt信号通路对脊髓损伤大鼠内源性神经干细胞增殖分化影响。方法:采用Allen s法制备脊髓损伤大鼠模型。分为假手术组、模型组、Wnt抑制剂组、烙灸组、烙灸联合Wnt抑制剂组,取损伤大鼠脊髓组织。采用运动神经功能评分(CBBB评分)、免疫组化、免疫荧光法检测脊髓组织内源性神经干细胞、神经元细胞及星形胶质细胞的标记物。结果:BBB评分、免疫组化、免疫荧光法检测结果均显示烙灸组最显著,Wnt抑制剂组与模型组差异无统计学意义。结论:烙灸激活脊髓损伤大鼠Wnt信号通路,促进其内源性神经干细胞增殖分化为神经元细胞,抑制分化为星形胶质细胞,使其神经细胞修复再生。

Objective To explore the effect of lao moxibustion mediated Wnt signal pathway on the proliferation and differentiation of endogenous neural stem cells in rats with spinal cord injury.Methods Allen s method was used to prepare the rat model of spinal cord injury.They were were divided into sham operation group,model group,Wnt inhibitor group,lao moxibustion group,lao moxibustion+Wnt inhibitor group.Basso Beattie Bresnahan(BBB)score,immunohistochemistry and immunofluorescence were used to detect the markers of endogenous neural stem cells,neuron cells and astrocytes in spinal cord tissue.Results BBB score,immunohistochemistry and immunofluorescence test results all showed that lao moxibustion group was the most significant,and there was no significant difference between the Wnt inhibitor group and the model group.Conclusion Lao moxibustion activated Wnt signaling pathway in rats with spinal cord injury,promoted the proliferation and differentiation of endogenous neural stem cells into neurons,inhibited the differentiation into astrocytes,and made the repair and regeneration of nerve cells.