石椒草化学成分研究

Chemical composition of Boenninghausenia sessilicarpa

采用硅胶、ODS、Sephadex LH-20和制备型高效液相等色谱方法对石椒草属植物石椒草Boenninghausenia sessilicarpa全草乙醇提取物的石油醚萃取部位进行分离纯化,同时根据化合物波谱数据对单体化合物进行结构鉴定。从石油醚萃取部位共分离鉴定了15个香豆素类化合物,结构鉴定分别为:欧芹烯酮酚甲醚(1)、九里香素(2)、3(-1,1-二甲基烯丙基)-8-羟基-7-甲氧基香豆素(3)、花椒毒素(4)、异茴芹素(5)、状芸香素(6)、异去甲基呋喃羽叶芸香素(7)、欧前胡素(8)、珊瑚菜内脂(9)、白芷脑素(10)、白当归素(11)、新比克白芷内酯(12)、chalepin(13)、鲁望桔内酯(14)、3(-1,1-二甲基烯丙基)-花椒内酯(15)。化合物1~3、6~10和14~15均首次从石椒草中分离得到。

The petroleum ether fraction of ethanol extracts from Boenninghausenia sessilicarpawas isolated by combination of several chromatographic methods including silica gel,ODS,and Sephadex LH-20 column chroma⁃tography,and finally purified by preparative HPLC.The structures of the isolated compounds were identified based on the spectral data.As a result,15 coumarin compounds were isolated and identified from the petroleum ether extraction.Their structures were determined as osthenon(1),murrangatin(2),3-(1,1-dimethylallyl)-8-hydroxy-7-methoxycoumarin(3),xanthotoxin(4),isopimpinellin(5),chalepensin(6),isodemethylfuropinarine(7),imperatorin(8),phellopterin(9),heraclenol(10),byakangelicin(11),neobyakangelicol(12),chalepin(13),luvan⁃getin(14),3-(1,1-dimethylallyl)-xanthyletin(15).Among them,compounds 1-3,6-10 and 14-15 were firstly isolated from B.sessilicarpa.