大黄芩鱼散半仿生提取物对鹅沙门氏菌生长的影响

Effects of semi-bionic extract of Dahuang Qinyu San on the growth of Salmonella goose in vitro

本研究旨在探讨大黄芩鱼散半仿生提取物(SEDQS)对鹅沙门氏菌的体外抗菌活性。采用微孔-平板法测定最小抑菌浓度(MIC),分光光度法和试剂盒法测定不同浓度SEDQS对鹅沙门菌生长曲线、呼吸代谢系统、可溶性蛋白的影响。结果显示:SEDQS对鹅沙门菌的MIC为(9.10±0.11)mg/mL,1/4 MIC~2 MIC的SEDQS均能显著抑制鹅沙门氏菌的正常生长,且最高抑制率为68.4%;能显著升高碱性磷酸酶(AKP)的含量(P<0.01),升高率达到88.2%;使细菌的核酸、蛋白质等大分子物质显著泄漏(P<0.01),且最高泄漏率为92%;还能显著地抑制琥珀酸脱氢酶(SDH)和苹果酸脱氢酶(MDH)的活性(P<0.01),并呈现剂量依赖性。由此表明,SEDQS通过破坏鹅沙门氏菌的细胞壁和细胞膜结构,影响其呼吸代谢而发挥体外抗菌活性。

The aim of this study was to investigate the antibacterial activity of the semi-bionic extract of Dahuang Qinyu San(SEDQS)against Salmonella goose(S.goose)in vitro.The minimum inhibitory concentration(MIC)of SEDQS against S.goose was determined by the micropore-plate method.The effects of SEDQS on the growth curve,respiratory metabolic system and soluble protein of S.goose were studied by the spectrophotometer and the kit method.The results showed that MIC of SEDQS against S.goose was(9.10±0.11)mg/mL and 1/4MIC~2MIC of SEDQS could significantly inhibit the growth of S.goose and the highest inhibition rate was 68.4%.The 1/4MIC~2MIC of SEDQS could significantly increased the content of AKP with an increase rate of 88.2%.The 1/4MIC~2MIC of SEDQS could significantly release nucleic acids and proteins from S.goose(P<0.01),and the highest leakage rate was 92%.The 1/4MIC~2MIC of SEDQS could significantly inhibited the activities of succinate dehydrogenase acid(SDH)and malate dehydrogenase acid(MDH)in a dose-dependent manner(P<0.01).The conclusion indicated that SEDQS exerted its antibacterial activity in vitro by destroying the cell wall and membrane structure of S.gosse and affecting its respiratory metabolism in vitro.