荔枝叶药材质量标准研究

Study on Quality Standard of the Leaves of Litchi Chinensis Sonn.

目的 建立荔枝叶药材的质量标准。方法 建立荔枝叶药材的性状、显微特征、薄层鉴别及含量测定方法;按照中国药典2020年版第四部通则中相关方法,对荔枝叶药材的水分、总灰分、酸不溶性灰分及浸出物等项目进行测定。结果 荔枝叶药材在性状、显微特征和薄层色谱方面均具有专属性特征。药材水分含量为2.66%~6.42%,总灰分含量为2.96%~4.52%,酸不溶性灰分含量为0.17%~0.94%,水溶性浸出物含量为15.38%~19.87%,醇溶性浸出物含量为24.94%~30.33%。没食子酸、原儿茶酸、儿茶素、表儿茶素、芦丁、紫云英苷、槲皮素、山柰酚8种成分分别在0.003 3~0.052 0 mg·mL^(–1)、0.006 9~0.109 6 mg·mL^(–1)、0.013 0~0.208 0 mg·mL^(–1)、0.057 5~0.920 0 mg·mL^(–1)、0.013 4~0.213 6 mg·mL^(–1)、0.013 2~0.211 2 mg·mL^(–1)、0.002 9~0.045 6 mg·mL^(–1)、0.003 6~0.056 8 mg·mL^(–1)内呈良好的线性关系(r>0.999),平均加样回收率97.43%~102.97%,RSD为1.70%~2.90%。结论 建立的荔枝叶药材质量标准具有很好的专属性、准确性、重现性,可用于荔枝叶药材的质量控制研究。

OBJECTIVE To establish the quality standard of the leaves of Litchi chinensis Sonn..METHODS The identification of medicinal properties,microscopic characteristics,TLC and content determination method of the leaves of Litchi chinensis Sonn.was conducted.The moisture,total ash,acid insoluble ash and extract of the leaves of Litchi chinensis Sonn.were determined according to Chinese Pharmacopoeia(Volume IV),2020.RESULTS The leaves of Litchi chinensis Sonn.under forest exhibited specific properties in characteristics,microscopic features and TLC results.The moisture content of the medicinal materials was 2.66%-6.42%,the total ash content was 2.96%-4.52%,the acid insoluble ash content was0.17%-0.94%,the water soluble extract was 15.38%-19.87%,and the alcohol soluble extract was 24.94%-30.33%.The eight components of gallic acid,protocatechin,catechin,epicatechin,rutin,astragalin,quercetin and kaempferol had a good linear relationship in 0.003 3-0.052 0 mg·mL^(–1),0.006 9-0.109 6 mg·mL^(–1),0.013 0-0.208 0 mg·mL^(–1),0.057 5-0.920 0 mg·mL^(–1),0.013 4-0.213 6 mg·mL^(–1),0.013 2-0.211 2 mg·mL^(–1),0.002 9-0.045 6 mg·mL^(–1),0.003 6-0.056 8 mg·mL^(–1)(r>0.999).The average sample recovery rate was 97.43%-102.97%and the RSD was 1.70%-2.90%.CONCLUSION The established quality standard of the leaves of Litchi chinensis Sonn.medicinal material has good specificity,accuracy and reproducibility,which can be used for quality control research of the leaves of Litchi chinensis Sonn..