高效液相色谱法测定玳玳花中柚皮苷和新橙皮苷含量

Content Determination of Naringin and Neohesperidin in Citri Aurantii Flos by HPLC

目的建立测定玳玳花中柚皮苷和新橙皮苷含量的高效液相色谱法。方法色谱柱为Akasil C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(20∶80,V/V),流速为1.0 mL/min,检测波长为283 nm,柱温为30℃,进样量为10μL。结果柚皮苷和新橙皮苷进样量分别在0.1104~1.7664μg、0.1181~1.8900μg范围内与峰面积积分值线性关系良好(r=1.0000,n=5);平均加样回收率分别为97.85%和98.43%,RSD分别为2.95%和2.22%(n=6)。13批样品中柚皮苷和新橙皮苷含量分别为2.85%~4.18%和8.05%~9.36%(n=3)。结论所建立的方法操作简便、结果准确,可用于玳玳花的质量控制。

Objective To establish a high-performance liquid chromatographic method for the content determination of naringin and neohesperidin in Citri Aurantii Flos.Methods The chromatographic column was Akasil C_(18)column(250 mm×4.6 mm,5µm),the mobile phase was acetonitrile-0.1%phosphoric acid solution(20∶80,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 283 nm,the column temperature was 30℃,and the injection volume was 10µL.Results The linear range of naringenin and neohesperidin were 0.1104-1.7664µg and 0.1181-1.8900µg(r=1.0000,n=5),respectively.The average recoveries of naringenin and neohesperidin were 97.85%and 98.43%with RSDs of 2.95%and 2.22%(n=6),respectively.The contents of naringin and neohesperidin in the 13 batches of samples were in the ranges of 2.85%-4.18%and 8.05%-9.36%(n=3),respectively.Conclusion The established method is simple,reliable and accurate,which can be used for quality control of Citri Aurantii Flos.