孕妇孕期血清维生素A、D和E的水平变化趋势研究

The trend of changes in serum vitamin A,D,and E levels during pregnancy

目的采用高效液相色谱-串联质谱(HPLC-MS/MS)定量检测孕妇孕中期维生素浓度,探究孕妇孕期血清维生素A、D和E的水平变化趋势。方法纳入2021年10月1日至2022年10月31日武汉大学人民医院产科建档720例孕妇,年龄(29.7±4.4)岁,孕期12~22周。采用HPLC-MS/MS测定维生素A、D和E的含量,统计分析各分组的浓度水平,并计算缺乏率。结果维生素A、D和E分布范围(95%CI):0.74~2.74μmol/L、2.88~25.37 ng/ml和6.18~35.08μmol/L,缺乏率分别为9.30%、93.76%和35.83%,双胎组的维生素A、D和E水平分别为(1.67±0.51)μmol/L、(13.18±7.44)ng/ml和11.97(8.85,14.60)μmol/L,均高于单胎组(1.45±0.36)μmol/L、(10.87±5.26)ng/ml和10.46(6.99,14.11)μmol/L,独立样本t检验有统计学意义(P<0.001)。VitD在体重指数较低组(<22 kg/m2)浓度为(12.54±5.74)ng/ml,高于BMI较高组(≥22 kg/m2)(10.46±4.90)ng/ml,差异有统计意义(P<0.001)。结论本研究使用HPLC-MS对孕中期3种维生素水平进行监测,监测孕期血清维生素A、D和E的水平变化。

Objective High performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was used to quantify the levels of vitamins A,D and E in pregnant women during the second trimester,and to investigate the change trends of serum vitamins A,D and E levels during pregnancy.Method A total of 720 pregnant women with an average age of(29.7±4.4)years and 12-22 weeks of gestation were included from October 1,2021 to October 30,2022 in the obstetrics department of the People′s Hospital of Wuhan University.The concentrations of vitamins A,D and E were determined by HPLC-MS/MS.The concentration levels of each group were statistically analyzed and the deficiency rate were calculated.Results The distribution range of vitamin A,D and E(95%CI)was 0.74-2.74μmol/L,2.88-25.37 ng/ml and 6.18-35.08μmol/L,with the deficiency rates were 9.30%,93.76%and 35.83%,respectively.Vitamin A,D and E levels in the twin group were(1.67±0.51)μmol/L,(13.18±7.44)ng/ml and 11.97(8.85,14.60)μmol/L,respectively.They were significantly higher than those in the singlet group(1.45±0.36)μmol/L,(10.87±5.26)ng/ml and 10.46(6.99,14.11)μmol/L,with statistical significance by independent sample t-test(P<0.001).The concentration of vitamin D in the lower BMI group(<22 kg/m2)was(12.54±5.74)ng/ml,significantly higher than that in the fat group(≥22 kg/m2)(10.46±4.90)ng/ml,and the rank-sum test was statistically significant(P<0.001).Conclusion In this study,the levels of three vitamins were monitored in mid-pregnancy using HPLC-MS/MS,and the changes of serum vitamin A,D,and E levels during pregnancy were analyzed.