宫颈癌组织中微小RNA-205、微小RNA-24-3p表达量及其与预后的关联性分析

Analysis of the expression levels of microRNA-205 and microRNA-24-3p in cervical cancer tissues and their correlation with survival prognosis

目的分析宫颈癌组织中微小RNA(miR)-205、miR-24-3p表达量及其与预后的关联性。方法回顾性选取2014年3月至2015年3月在湖北省中西医结合医院治疗的宫颈癌患者82例(宫颈癌组)、子宫肌瘤患者60例(子宫肌瘤组)和同期接受宫颈癌体检的健康女性40例(健康对照组),检测三组组织中miR-205、miR-24-3p表达量,采用受试者工作特征(ROC)曲线分析miR-205、miR-24-3p对宫颈癌的诊断价值,对宫颈癌患者随访5年,分析影响宫颈癌患者预后的危险因素。结果宫颈癌组miR-205、miR-24-3p相对表达量高于子宫肌瘤组及健康对照组(P<0.01)。ROC曲线分析结果表明,组织中miR-205、miR-24-3p水平的截断值为1.13和1.35时,曲线下面积最大,诊断宫颈癌的灵敏度分别为83.87%、84.38%,特异度分别为75.00%、82.42%。生存分析结果显示,miR-205≥1.13患者5年生存率为69.05%,明显低于miR-205<1.13患者的87.50%(P<0.05);miR-24-3p≥1.35患者5年生存率为68.89%,明显低于miR-24-3p<1.35患者的89.19%(P<0.05)。Cox回归结果显示,中高分化程度、国际妇产科联合会分期Ⅱ期、浸润深度≥1/2、合并淋巴结转移及miR-205、miR-24-3p高水平为影响宫颈癌患者预后的危险因素(P<0.05)。结论miR-205、miR-24-3p在宫颈癌组织中呈较高水平,且与患者预后关系密切,有望成为宫颈癌的辅助诊断和预后评估的指标之一。

Objective To analyze the expression of microRNA(miR)-205 and miR-24-3p in cervical cancer tissues and their correlation with survival prognosis.Methods A total of 82 patients with cervical cancer(cervical cancer group),60 patients with uterine fibroids(uterine fibroids group)and 40 healthy women(healthy control group)treated in Hubei Hospital of Integrated Traditional Chinese and Western Medicine from March 2014 to March 2015 were enrolled.The expression levels of miR-205 and miR-24-3p in the tissues of the three groups were compared.The receiver operative characteristic(ROC)curve was used to analyze the diagnostic value and zero threshold of the two indicators for cervical cancer.Analyzed the 5-year survival status of cervical cancer patients at the upper and lower thresholds,and then further discuss the risk factors that affected the prognosis of cervical cancer.Results The relative expression levels of miR-205 and miR-24-3p in the cervical cancer group were significantly higher than those in the uterine fibroids group and the healthy control group(P<0.01).ROC curve analysis showed that the cut-off value was 1.13 and 1.35,the area under the curve was the biggest,and the sensitivity in diagnosis of cervical cancer were 83.87%,84.38%,the specificity were 75.00%,82.42%.The survival rate analysis based on these results showed that the 5-year survival rate of patients with miR-205≥1.13 was 69.05%,which was significantly lower than patients with miR-205<1.13(87.50%)(P<0.05);the 5-year survival rate of patients with miR-24-3p≥1.35 was 68.89%,which was significantly lower than that of patients with miR-24-3p<1.35(89.19%)(P<0.05).The results of the Cox regression model showed that moderately and highly differentiated,FIGO stageⅡ,depth of invasion≥1/2,combined lymph node metastasis,and high levels of miR-205 and miR-24-3p were risk factors affecting the prognosis of cervical cancer patients(P<0.05).Conclusions Serum miR-205 and miR-24-3p are at high levels in cervical cancer tissues,and are closely related to the prognosis of patients.They are expected to become auxiliary diagnosis and prognostic indicators for cervical cancer.