毛栓孔菌XYG422产木质素降解酶的固体发酵工艺研究

Solid state fermentation technology of lignin degrading enzyme produced by Trametes hirsuta XYG422

为了提高动物粗饲料的消化率,研究以黄芩药渣为基质,采用单因素和正交试验对毛栓孔菌XYG422产木质素降解酶发酵条件进行优化。结果显示,毛栓孔菌XYG422固体发酵的最佳产酶培养基组成为可溶性淀粉∶硫酸铵3∶1、Cu^(2+)浓度3 mmol/L、Mn^(2+)浓度1.8 mmol/L。在此条件下,锰过氧化物酶活力为179.37 nmol/(min·g),漆酶活力为41.68 U/g。研究结果对毛栓孔菌木质素降解酶的生产与应用具有一定的指导意义,可为开发提高纤维消化率的饲料提供借鉴。

In order to improve the digestibility of animal coarse feed,the fermentation conditions of lignin degrading enzyme production by Trametes hirsuta XYG422 were optimized by user of single factor and orthogonal experiments.Scutellaria residue was used as the substrate.The results showed that the optimized medium composition for solid fermentation of Trametes hirsuta XYG422 was as followed:Soluble starch∶ammonium sulfate 3∶1,3 mmol/L Cu^(2+)and 1.8 mmol/L Mn^(2+).Under the conditions,manganese peroxidase activity reached 179.37 nmol/(min·g),laccase activity reached 41.68 U/g.The research has certain guiding significance for the production and application of Trametes hirsuta,and also provides novel opportunities to develop livestock feeds with improved fiber digestibility.