绿茶、乌龙茶和红茶提取物对2,4-二氯苯氧乙酸诱导的斑马鱼胚胎形态发育毒害的影响

Effects of green,Oolong,and black tea extracts on 2,4-dichlorophenoxyacetic acid-induced embryonic morphological developmental toxicity of zebrafish

目的研究绿茶提取物(green tea extract,GTE)、乌龙茶提取物(Oolong tea extract,OTE)和红茶提取物(black tea extract,BTE)对2,4-二氯苯氧乙酸(2,4-dichlorophenoxyacetic acid,2,4-D)导致的斑马鱼胚胎发育毒害的影响。方法用50 mg/L 2,4-D分别联合6.25、12.50和25.00 mg/L GTE,6.25、12.50和25.00 mg/L OTE以及50、100和150 mg/L BTE处理斑马鱼胚胎至96 hpf,体视显微镜观察斑马鱼胚胎发育并统计孵化率、死亡率、畸形率,测定斑马鱼心跳次数、体长、卵黄和心包面积。结果各处理组斑马鱼胚胎孵化正常。除2,4-D+BTE处理组随BTE浓度提高死亡率升高,其他处理组没有发生显著死亡。GTE降低2,4-D导致的斑马鱼胚胎畸变率,提高2,4-D导致的心跳下降和体长缩短,缩小2,4-D导致的卵黄和心包面积;相反,BTE加重2,4-D导致的斑马鱼胚胎形态发育毒害;而OTE对2,4-D导致的斑马鱼胚胎发育毒害无明显影响。结论GTE、OTE和BTE对2,4-D导致的斑马鱼胚胎发育毒害分别具有拮抗作用、无作用和协同作用。

Objective To investigate the effects of green tea extract(GTE),Oolong tea extract(OTE)and black tea extract(BTE)on the developmental toxicity of zebrafish embryos caused by 2,4-dichlorophenoxyacetic acid(2,4-D).Methods Zebrafish embryos were treated with 50 mg/L 2,4-D in combination with 6.25,12.50 and 25.00 mg/L GTE,6.25,12.50 and 25.00 mg/L OTE and 50,100 and 150 mg/L BTE up to 96 hpf,and zebrafish embryos were observed for hatching rate,mortality rate,malformation rate the number of heartbeats,body length,yolk and pericardial area of zebrafish were also measured.Results Zebrafish embryos hatched normally in all treatment groups.No significant mortality occurred in the treatment groups except for the 2,4-D+BTE treatment group where mortality increased with increasing BTE concentration.GTE reduced the rate of 2,4-D-induced malformation in zebrafish embryos,increased 2,4-D-induced heartbeat reduction and body length shortening,and reduced 2,4-D-induced yolk and pericardial area.Conversely,BTE increased 2,4-D-induced morphological toxicity in zebrafish embryos,whereas OTE had no significant effect on the developmental toxicity of 2,4-D-induced zebrafish embryos.Conclusion GTE,OTE and BTE have antagonistic,no effect and synergistic effects on the developmental toxicity of zebrafish embryos caused by 2,4-D,respectively.