子宫内膜癌组织的CRYAB水平及临床意义

Expression and clinical significance of CRYAB in endometrial cancer tissues

目的探讨子宫内膜癌组织中αB-晶状体蛋白(CRYAB)的水平并结合组织病理学特征和手术治疗方案分析该指标的预后价值。方法收集本院2015年1月至2016年1月完成诊治和随访的78例子宫内膜癌患者的癌组织和癌旁组织,采用实时定量PCR(qPCR)检测CRYAB水平,分析CRYAB水平与子宫内膜癌临床病理特征的关系,生存分析采用Kaplan-Meier法,Cox比例风险模型研究影响预后的独立风险因素。结果子宫内膜癌组织的CRYAB水平为0.974±0.054,低于癌旁组织的1.347±0.066,差异有统计学意义(t=4.361,P<0.001),进一步分析发现CRYAB水平与病理分期、分化程度、肌层浸润深度和淋巴结转移有关(P<0.05),其中CRYAB水平在病理分期晚、低分化、深肌层浸润及淋巴结转移子宫内膜癌组织中降低。生存分析显示年龄、手术方式、病理分期、病理类型、分化程度、肌层浸润深度、辅助治疗方案对患者的术后生存无显著影响,而CRYAB水平降低(HR=7.156,95%CI:2.412~21.579,P=0.016)和淋巴结转移(HR=5.360,95%CI:1.045~27.488,P=0.044)是影响患者术后生存的独立危险因素。结论异常低表达的CRYAB水平在子宫内膜癌进展中起着重要作用,可能是子宫内膜癌的独立诊断和预后生物标志物。

Objective To explore the level of α-B-Crystallin(CRYAB)in endometrial cancer tissues and analyze its prognostic value based on histopathological characteristics and surgical treatment protocols.Methods The cancer tissues and adjacent tissues from 78 patients with endometrial cancer who completed diagnosis,treatment and follow-up in our hospital from January 2015 to January 2016 were collected.Real-time quantitative PCR(qPCR)was used to detect CRYAB levels and relationship between CRYAB levels and clinical pathological characteristics of endometrial cancer were analyzed.Survival analysis was conducted using Kaplan-Meier method and Cox proportional risk model was applied to study independent risk factors affecting prognosis.Results The CRYAB level in endometrial cancer tissues was 0.974±0.054,lower than 1.347±0.066 in adjacent tissues with a statistically significant difference(t=4.361,P<0.001).Further analysis found that the CRYAB level was related to pathological stage,differentiation degree,depth of myometrial invasion,and lymph node metastasis(P<0.05),with a decrease in CRYAB level in endometrial cancer tissues with late pathological stage,poor differentiation,deep stromal invasion and lymph node metastasis.Survival analysis showed that age,surgical method,pathological stage,pathological type,degree of differentiation,depth of muscle infiltration,and adjuvant treatment regimen had no significant impact on postoperative survival of patients.However,decreased CRYAB level(HR=7.156,95%CI:2.412-21.579,P=0.016)and lymph node metastasis(HR=5.360,95%CI:1.045-27.488,P=0.044)were independent risk factors affecting postoperative survival of patients.Conclusion The abnormally low expression of CRYAB plays an important role in the progression of endometrial cancer and may be an independent diagnostic and prognostic biomarker for endometrial cancer.