酶法生产L-高苯丙氨酸的研究进展

Advances in enzymatic production of L-homophenylalanine

L-高苯丙氨酸(L-homophenylalanine,L-HPA)作为一种重要的非天然氨基酸,是合成治疗高血压的普利类药物等的关键中间体,具有广阔的市场前景。目前L-高苯丙氨酸的合成主要依赖于化学法,但化学合成L-高苯丙氨酸具有原料昂贵、步骤繁琐和污染严重等缺点,限制了广泛应用。因此,国内外研究者对L-高苯丙氨酸的酶法生产进行了深入的研究。本文就目前酶法合成L-高苯丙氨酸的工艺,包括脱氢酶法、转氨酶法、海因酶法和脱羧酶法的研究进展进行了综述,为酶法合成L-高苯丙氨酸提供一定的借鉴,为最终实现L-高苯丙氨酸的酶法工业化生产奠定基础。

L-homophenylalanine(L-HPA)is an important non-natural amino acid that has been used as a key intermediate for the synthesis of Puli drugs for the treatment of hypertension.At present,L-HPA is synthesized using chemical methods,which has the disadvantages of expensive raw materials,tedious steps and serious pollution.Therefore,researchers have conducted in-depth research on the enzymatic production of L-HPA.This review summarizes the research progress on the enzymatic synthesis of L-HPA,including the dehydrogenase process,the transaminase process,the hydantoinase process,and the decarboxylase process,with the hope to facilitate the industrial production of L-HPA.