杞参方调控高渗诱导的干眼模型小鼠MAPK通路机制

Study on the Protective Mechanism of Qishen Prescription on MAPK Pathway in Hypertonic Induced Dry Eye Model Mice

目的:基于MAPK通路观察杞参方对高渗诱导的干眼模型小鼠的效应。方法:实验研究。2021年1月将60只BALB/c小鼠按照随机数字表法分为对照组、模型组、西药组以及杞参方颗粒高、中、低剂量组,每组10只。对照组不做任何处理,其余各组小鼠使用高渗盐水建立干眼模型。成模后,模型组继续高渗盐水点眼;西药组给予高渗盐水联合玻璃酸钠滴眼液点眼;杞参方高、中、低剂量组分别给予高渗盐水点眼联合杞参方4.80、2.40、1.20 g·kg^(-1)·d^(-1)灌胃,1次/d。给药14 d后测定泪液分泌试验(SⅠT)、泪膜破裂时间(BUT)、角膜荧光素染色(FL);电镜观察角膜上皮细胞超微结构;Western Blot分别检测角膜组织中c-Jun氨基末端激酶1(JNK1)、磷酸化-JNK1(p-JNK1)、p38丝裂原活化蛋白激酶(p38MAPK)、磷酸化p38丝裂原活化蛋白激酶(p-p38)、细胞外调节蛋白激酶1(ERK1)、磷酸化-ERK1(p-ERK1)蛋白表达。多组间数据比较采用单因素方差分析,两两比较采用LSD-t检验。结果:与对照组相比,模型组和各给药组小鼠SⅠT、BUT、FL均明显受到损害(P<0.01),角膜组织中p-JNK1、p-p38、p-ERK1表达均显著增高(P<0.01)。与模型组相比,给药组SⅠT、FL、BUT均有改善(P<0.05),各用药组的p-JNK1及杞参方高、中剂量组的p-p38、p-ERK1表达均减少(P<0.05)。与西药组相比,杞参方高剂量组的SⅠT增加(P=0.048),杞参方高、中剂量组FL有所改善(P=0.004、0.014),杞参方高剂量组的p-JNK1、p-p38、p-ERK1表达减少(P=0.017、0.003、0.001)。电镜显示杞参方各用药组角膜上皮微绒毛数量及规则程度要优于模型组与西药组。结论:杞参方可有效改善高渗诱导干眼模型小鼠的SⅠT、BUT、FL及角膜上皮细胞微绒毛形态,减少角膜组织JNK1、p38MAPK、ERK1蛋白的磷酸化表达。

Objective:To observe the protective effect of Qishen prescription(QSF)on dry eye(DE)model mice induced by hypertonic fluid.Methods:In this experimental study,in January 2021,60 BALB/c mice were selected and randomly divided into 6 groups according to the random number table method,which were named as control group,model group,western medicine group,and QSF low-dose,medium-dose and highdose groups,with 10 mice in each group.Except for the control group,the DE mice model was established with hypertonic saline solution.After establishing the successful model,the model group continued to receive hypertonic saline solution to maintain DE.QSF high-dose,medium-dose and low-dose groups were administered hypertonic saline solution eye drops combined with QSF granules through intragastric administration once a day.Western medicine group was given hypertonic saline combined with 0.3%sodium hyaluronate eye drops.All intervention factors were applied continuously for 14 days.Schirmer test(S T),breakup time(BUT)and Fluorescent(FL)were measured.Then,the ultrastructure of corneal epithelial cells was observed by transmission electron microscopy.The protein expression levels of JNK1,p-JNK1,p38MAPK,p-p38MAPK,ERK1 and p-ERK1 in corneal tissues were measured by Western Blot.One way ANOVA analysis was used for inter group comparison,and LSD-t test was used for pairwise comparison.Results:Compared with the control group,the S T,BUT and FL was significantly different in each group(P<0.01).The protein expression levels of p-JNK1,p-p38,p-ERK1 were significantly increased in each group(P<0.01).Compared with the model group,the administration group showed improvement in S T,FL,and BUT(P<0.05),while the p-JNK1 expression in each medicine group and the p-p38 and p-ERK1 in the high and medium dose groups of QSF decreased(P<0.05).Compared with the positive medicine group,the high-dose group of QSF exhibited an increase in S T(P=0.048),while the high and medium dose groups of QSF showed improvements in FL(P=0.004,0.014),and the high-dose group of QSF showed a decrease in the expression of p-JNK1,p-p38,and p-ERK1(P=0.017,0.003,0.001).Electron microscope showed that the number and regularity of corneal epithelial microvilli in each treatment group of QSF were better than those in the model group and western medicine group.Conclusions:QSF can not only effectively improve S T,BUT,FL,corneal epithelial cell microvilli morphology of hypertonicinduced dry eye in mice,but also reduce the phosphorylation of JNK1,p38MAPK,ERK1.