系统性红斑狼疮患者长链非编码RNA表达变化及其与CD8^(+)T细胞相关性研究

Changes of lncRNA expression and its correlation with CD8^(+)T cells in patients with systemic lupus erythematosus

目的探讨系统性红斑狼疮(SLE)患者长链非编码RNA(lncRNA)的表达变化及其与CD8^(+)T细胞数量和疾病活动的关系。方法选择2020年7月至12月在山西医科大学第二医院风湿免疫科住院的SLE患者9例,选取同期本院健康体检中心的9例健康者为对照组。从外周血单个核细胞中提取RNA,用全转录组测序分析lncRNA表达谱,筛选差异表达的lncRNA。比较两组CD8^(+)T细胞计数,并分析差异表达的lncRNA与CD8^(+)T细胞数量及SLE患者临床特征的相关性。结果与健康对照组比较,SLE患者有240个差异表达lncRNA,其中134个高表达,106个低表达,并且AL450352.1(r=0.755,P=0.030)、LINC00944(r=0.824,P=0.012)的表达量与外周血CD8^(+)T细胞计数呈正相关,PSORS1C3(r=-0.784,P=0.021)、TDRG1(r=-0.808,P=0.015)、MIR3150BHG(r=-0.707,P=0.049)、AC000403.1(r=-0.726,P=0.040)、AL034550.2(r=-0.762,P=0.028)、AC099524.1(r=-0.714,P=0.047)、HOMER3-AS1(r=-0.760,P=0.029)的表达量与外周血CD8^(+)T细胞计数呈负相关。其中HOMER3-AS1与SLE患者24 h尿蛋白呈正相关(r=0.774,P=0.024)。结论SLE患者lncRNA表达谱发生变化,部分差异表达的lncRNA与CD8^(+)T细胞数量相关,并可能通过影响SLE患者CD8^(+)T细胞数量进而影响SLE患者肾脏功能。

Objective To explore the relationship between differential long non-coding RNA(lncRNA)expressions,the number of CD8^(+)T cells,and disease activity in systemic lupus erythematosus(SLE)patients.Methods From July to December 2020,9 hospitalized SLE patients at the Rheumatology and Immunology Department of Shanxi Medical University′s Second Hospital were chosen,and 9 healthy patients from the hospital′s physical examination center were chosen as the healthy control group at the same time.To identify differentially expressed lncRNA,peripheral blood mononuclear cells were collected and their lncRNA expression patterns were evaluated using whole transcriptome sequencing.CD8^(+)T cell count in the two groups was compared,and the relationships between differentially expressed lncRNA and CD8^(+)T cell count and clinical characteristics in SLE patients were investigated.Results SLE patients had 240 differently expressed lncRNAs when compared to healthy control group,including 134 highly expressed lncRNAs and 106 weakly expressed lncRNAs.The expression of AL450352.1(r=0.755,P=0.030)and LINC00944(r=0.824,P=0.012)were positively correlated with the number of CD8^(+)T cells,and the expression of PSORS1C3(r=-0.784,P=0.021),TDRG1(r=-0.808,P=0.015),MIR3150BHG(r=-0.707,P=0.049),AC000403.1(r=-0.726,P=0.040),AL034550.2(r=-0.762,P=0.028),AC099524.1(r=-0.714,P=0.047),HOMER3-AS1(r=-0.760,P=0.029)were negatively correlated with the number of CD8^(+)T cells.HOMER3-AS1,which was negatively correlated with peripheral blood CD8^(+)T cells,was positively correlated with 24-hour proteinuria in patients with SLE(r=0.774,P=0.024).Conclusion The expression profile of lncRNAs changes in SLE patients,and certain differentially expressed lncRNAs are connected with the number of CD8^(+)T cells in SLE patients,which may further impair SLE patients′kidney function by influencing the number of CD8^(+)T cells.