“短时取样法”用于非侵入性胚胎植入前遗传学检测效能的前瞻性研究

Efficiency of"short time sampling"in noninvasive preimplantation genetic testing:a prospective study

目的探讨"短时取样法"在非侵入性胚胎植入前遗传学检测(non-invasive preimplantation genetic testing,ni-PGT)中的检测效能。方法采用前瞻性研究分析方法,对2021年3月至2022年6月期间就诊于湘潭市中心医院生殖中心行胚胎植入前非整倍体遗传学检测(preimplantation genetic testing for aneuploidy,PGT-A)患者的囊胚进行外滋养层细胞(trophectoderm,TE)活检,同时收集囊胚培养液(spent culture medium,SCM)。根据SCM收集方法的不同,分为直接收集法与"短时取样法",分析比较两种取样方法ni-PGT的检测结果与PGT-A结果的符合率。结果本研究收集36对夫妇的108个囊胚,均行TE活检及收集SCM,其中直接收集法59例、"短时取样法"49例。直接收集法诊断胚胎倍性的曲线下面积(area under curve,AUC,0.628)小于"短时取样法"(0.785)。结论"短时取样法"诊断胚胎倍性的效能相较于直接收集法更优。

Objective To explore the efficiency of"short time sampling"for noninvasive preimplantation genetic testing(ni-PGT).Methods A prospective study was conducted.The blastocysts of patients who were treated by preimplantation genetic testing for aneuploidy(PGT-A)in the Reproductive Center of Xiangtan Central Hospital from March 2021 to June 2022 were biopsied,meanwhile the spent culture medium(SCM)were collected.According to the different sampling methods for SCM,they were divided into two groups,direct sampling method and"short time sampling".The coincidence rate between ni-PGT and PGT-A was analyzed and compared statistically.Results Totally 108 blastocysts from 36 couples were biopsied and SCMs were collected,including 59 cases of direct collection and 49 cases of"short time sampling".The area under curve(AUC)of direct collection method for diagnosing embryo ploidy was lower than that of"short time sampling"(0.628 vs.0.785).Conclusion The efficiency of"short time sampling"in diagnosing embryo ploidy is better than that of direct collection method.