木瓜蛋白酶纳米簇荧光探针的汞离子检测及其在环境监测中的应用

Mercury Ion Detection of Papain-nanocluster Fluorescent Probe and Its Application in Environmental Monitoring

环境中的Hg2+具有强烈的毒性和高度的生物富集性,可被人体吸收且不易排出,因此对环境中Hg2+的灵敏检测具有重要意义。本文以绿色荧光的木瓜蛋白酶铂纳米簇(Papain-PtNCs)为荧光探针对16种不同金属离子进行检测,结果显示该探针对Hg2+具特异性。随后将不同浓度的Hg2+与Papain-PtNCs混合,发现该探针荧光强度与Hg2+浓度具有线性关系,检测范围为5~100μM,最低检测限达2.7μM,低于国家饮用水标准中规定的Hg2+含量(5μM)。进一步研究发现该纳米探针可成功应用于定量检测包括水样和土样在内的环境样品中的Hg2+,其回收率为95.82%~109.20%。由此可见,该荧光纳米探针可用于快速、便捷且经济地对环境中Hg2+进行检测,从而为重金属污染防治和环境监测领域提供一种有益的技术,并为其商业化应用提供了可能。

Hg2+in the environment has strong toxicity and high bio-enrichment,which can be absorbed by the hu-man body and is hard to discharge.Thus,it is of great significance for sensitive detection of Hg2+concentration in the environment.In this paper,green fluorescent papain-platinum nanoclusters(Papain-Pt NCs)were used as a kind of fluorescent probe for the detection of 16 different metal ions and the results showed that the probe was spe-cific for Hg2+.Subsequently,by mixing different concentrations of Hg2+with Papain Pt NCs,the fluorescence in-tensity of the bio-probe exhibited a linear relationship with Hg2+concentration,and its detection range and detec-tion limit were respectively 5-100μM,and 2.7μM,lower than the national standard of Hg2+(5μM).Further-more,the nanoprobe could be successfully applied to quantitative determination of Hg2+in environmental sam-ples,including water and soil samples,and the recovery rate was 95.82%~109.20%.Therefore,the fluorescent nanoprobe can be used to detect Hg2+in the environment quickly,conveniently and economically,which is antici-pated to provide a profitable technology for the prevention and control of heavy metal pollution and environmen-tal monitoring,as well as the possibility of its commercial application.