紫芝菌株Gs-1液体培养产漆酶的培养基优化研究

Optimization of the liquid medium for laccase production by Ganoderma sinense strain Gs-1

为确定紫芝菌株Gs-1产漆酶的最适液体培养基,以漆酶活力为指标,采用单因素试验研究碳源、氮源、金属离子和农副产品诱导物对紫芝菌株Gs-1产漆酶的影响,在单因素试验基础上采用Box-Behnken试验设计优化紫芝菌株Gs-1产漆酶的最适液体培养基条件。结果表明:优化的最适液体培养基条件为葡萄糖质量浓度19.21 g/L、胰蛋白胨质量浓度2.0 g/L、Cu^(2+)浓度1.08 mmol/L、农副产品诱导物豆粕质量浓度32.59 g/L。在此优化条件下,紫芝菌株Gs-1产漆酶的活力可达6787.58 U/L,是未优化培养基中漆酶活力的26.46倍。紫芝菌株Gs-1液体培养基的优化极大地提高了其胞外漆酶活力,研究结果为紫芝漆酶的工业化生产奠定了坚实基础。

In order to determine the optimal liquid medium for the secretion of laccase by Ganoderma sinense strain Gs-1 with submerged fermentation,the laccase activity of G.sinense strain Gs-1 with submerged fermentation was used as an index in this study,and single factor experiments were used to study the effects of carbon sources,nitrogen sources,metal ions,and agricultural by-products as inducers on laccase secretion by G.sinense strain Gs-1 with submerged fermentation.Then the Box-Behnken experimental design was used to optimize the liquid medium for the secretion of laccase by G.sinense strain Gs-1 with submerged fermentation.The results showed that the optimal carbon source for the secretion of laccase by G.sinense strain Gs-1 with submerged fermentation was glucose and the optimal concentration of glucose was 19.21 g/L;the optimal nitrogen source for the secretion of laccase by G.sinense strain Gs-1 with submerged fermentation was tryptone and the optimal concentration of tryptone was 2.0 g/L;the optimal metal ion for the secretion of laccase by G.sinense strain Gs-1 with submerged fermentation was Cu 2+and the optimal concentration of Cu^(2+)was 1.08 mmol/L;the optimal agricultural by-product for the secretion of laccase by G.sinense strain Gs-1 with submerged fermentation was soybean meal and the optimal concentration of soybean meal was 32.59 g/L.Under these optimized conditions,the activity of laccase secreted by G.sinense strain Gs-1 with submerged fermentation could reach 6787.58 U/L,which was 26.46 times of laccase activity secreted by G.sinense strain Gs-1 with submerged fermentation in the unoptimized medium.The optimization of G.sinense strain Gs-1 liquid medium greatly improved the activity of its extracellular laccase,and the results laid a solid foundation for the industrial production of G.sinense strain laccase.