宫颈癌患者血清Stathmin、Sox2及VEGF mRNA表达与淋巴结转移的相关性

Correlation between Serum Stathmin,Sox2 and VEGF mRNA Expression and Lymph Node Metastasis in Patients with Cervical Cancer

目的探讨宫颈癌患者血清Stathmin、Sox2及血管内皮生长因子(VEGF)mRNA表达与淋巴结转移的相关性。方法选取2017年1月—2020年2月收治的宫颈癌80例作为A组,另选取同期宫颈上皮内瘤样病变60例作为B组以及行子宫全切术的其他子宫良性病变60例作为C组。采用PT-PCR检测3组血清VEGF、Sox2、Stathmin mRNA表达情况;采用Pearson相关系数分析血清VEGF、Sox2、Stathmin mRNA表达与临床特征的相关性;采用多因素Logistic回归分析探讨宫颈癌患者淋巴结转移危险因素。结果A组血清VEGF、Sox2、Stathmin mRNA表达水平均高于B组和C组,且B组高于C组(P<0.05)。淋巴结转移宫颈癌患者血清VEGF、Sox2、Stathmin mRNA表达水平均高于无淋巴结转移患者(P<0.05)。血清VEGF、Sox2、Stathmin mRNA表达均与淋巴结转移、分化程度、国际妇产科学联合会(FIGO)分期呈显著正相关(P<0.01)。FIGO分期、分化程度、肿瘤直径及血清VEGF、Sox2、Stathmin mRNA表达为宫颈癌患者淋巴结转移的独立危险因素(P<0.01)。结论宫颈癌患者血清VEGF、Sox2和Stathmin mRNA表达上调,其表达水平与淋巴结转移密切相关。

Objective To investigate the correlation between serum Stathmin,Sox2,vascular endothelial growth factor(VEGF)mRNA expression and lymph node metastasis in patients with cervical cancer.Methods A total of 80 patients with cervical cancer admitted from January 2017 to February 2020 were selected as group A,60 patients with cervical intraepithelial neoplasia during the same period were selected as group B and 60 patients with other benign uterine lesions undergoing total hysterectomy were selected as group C.The mRNA expressions of VEGF,Sox2 and Stathmin in serum of the three groups were detected by PT-PCR.Pearson correlation coefficient was used to analyze the correlation between serum VEGF,Sox2 and Stathmin mRNA expression and clinical characteristics.Multivariate Logistic regression analysis was used to investigate the risk factors of lymph node metastasis in patients with cervical cancer.Results The mRNA expression levels of serum VEGF,Sox2 and Stathmin in group A were higher than those in groups B and C,and higher in group B than in group C(P<0.05).The mRNA expression levels of serum VEGF,Sox2 and Stathmin in patients with lymph node metastasis were higher than those in patients without lymph node metastasis(P<0.05).The mRNA expressions of serum VEGF,Sox2 and Stathmin were positively correlated with lymph node metastasis,differentiation degree,and International Federation of Gynecology and Obstetrics(FIGO)(P<0.01).FIGO stage,differentiation degree,tumor diameter and mRNA expression of serum VEGF,Sox2 and Stathmin were independent risk factors for lymph node metastasis in patients with cervical cancer(P<0.01).Conclusion The expression levels of serum VEGF,Sox2 and Stathmin mRNA in patients with cervical cancer are up-regulated,which are closely related to lymph node metastasis.