基于基因组重测序的野生板栗遗传特征和分类地位研究

Study on Genetic Characteristics and Taxonomic Status of Wild Chinese Chestnut Based on Genome Re-sequencing

利用SSR分子标记对全国范围内的194份板栗资源进行聚类分析,并对野生资源的植物学特征进行描述和对比。选出46份代表性的野生和栽培板栗资源进行重测序,对其进行系统发育和群体结构的分析。聚类结果显示,野生板栗与栽培板栗具有非常近的遗传距离,两者相互混杂,没有明显的独立群体划分;群体结构分析也表明野生板栗与栽培板栗具有一致的遗传信息,栽培板栗可能是野生板栗就地驯化而来且驯化时间有限。利用高通量测序技术明确了野生板栗的分类地位,即野生板栗与栽培板栗属于同一种,明确了成串结果习性并不是野生板栗的特性,提出了中国野生板栗更为准确和最新的边界范围。

The cluster analysis of 194 chestnut resources was performed based on SSR molecular markers,and the botanical characteristics of wild chestnut resources were described and compared.Forty-six representative wild and cultivated Chinese chestnut resources were selected for re-sequencing analysis,and phylogenetic and population structure analyses were performed.The clustering results showed that wild Chinese chestnuts and cultivated Chinese chestnuts had a close genetic distance,they were mixed and no obvious division;population structure analysis also further supported that wild Chinese chestnuts and cultivated Chinese chestnuts had the consistent genetic information,revealing cultivated Chinese chestnut may be domesticated from wild Chinese chestnut in situ for a limited time.This study clarified the taxonomic status of wild chestnut,namely:wild Chinese chestnut and cultivated Chinese chestnut belong to the same species.It is clear that the habit of female flower bunching is not a characteristic of wild Chinese chestnut,and a more accurate and up-to-date boundary range of wild Chinese chestnut is proposed.