表面等离体共振成像用于细菌分析与检测的研究进展

Progress in the application of surface plasmon resonanceimaging for bacterial analysis and detection

表面等离体共振成像(surface plasmon resonance imaging, SPRI)技术作为一种检测方法,具有快速灵敏、样品用量少、无损免标记和实时监测等优点,在生物分子研究方面已有广泛应用。细菌与人类关系紧密,对细菌的分析和检测在环境、农业、食品安全和医疗卫生方面具有重要意义。近年来,将SPRI技术应用于细菌的分析和检测,逐渐成为细菌研究领域的热点之一。对近10年来SPRI技术在细菌个体、细菌生物膜,以及细菌定性/定量分析与检测领域的具体研究进展进行综述,展示了SPRI技术在这些领域的良好应用前景,并对SPRI技术在相关领域的研究方向进行了展望。

As a detection method,Surface plasmon resonance imaging(SPRI)technology has the advantages of fast and sensitive,low sample consumption,real-time,label-free and non-invasion detection,and has been extensively employed in the biomolecular research fields.Bacteria are closely related to humans.Therefore,the analysis and detection of bacterial is of great significance in the fields of environment,agriculture,food safety,and medicine and health.Application of SRPI technology in the analysis and detection of bacterial has become one of hot spots in bacterial research areas in recent years.This review summarizes the research progress of SPRI technology for analysis of individual bacteria and biofilm,as well as qualitative and quantitative analysis and detection of bacteria in the last decade,and illustrates the good potential of the SPRI technology in these fields.The future research directions of SPRI technology in these fields were also mentioned.