响应面法优化南方红豆杉中10-DAB提取工艺及其含量测定

Response Surface Methodology for Optimization of Extraction Process and Content Determination of 10-DAB in Taxus wallichiana var.mairei

为探究南方红豆杉(Taxus wallichiana var.mairei)中10-去乙酰基巴卡亭Ⅲ(10-DAB)的最佳提取工艺,本研究以南方红豆杉中10-DAB含量为指标,以料液比、提取时间及超声温度为考察因素,采用单因素试验结合响应面法优化10-DAB的提取工艺,并采用超高效液相色谱(UPLC)法检测不同采摘部位、不同生长年限及不同采收时节的10-DAB含量差异。结果表明,南方红豆杉中10-DAB的最佳提取工艺条件为料液比1∶15(g/mL)、提取时间23 min、超声温度40℃,该工艺条件下10-DAB的含量为1.18%,与理论值相对偏差为0.42%。UPLC检测结果表明,南方红豆杉叶的10-DAB含量显著高于枝和树皮,且10-DAB全年累积含量受季节影响明显,10月份达到最高值。该研究可为南方红豆杉中10-DAB的提取利用提供参考依据。

In order to explore the optimal extraction process of 10-DeacetylbaccatinⅢ(10-DAB)from Taxus wallichiana var.mairei leaves,the content of 10-DAB in T.wallichiana var.mairei was taken as the index,and the solid-liquid ratio,extraction time and ultrasonic temperature were taken as the investigation factors.The extraction process of 10-DAB was optimized by single factor test combined with response surface method,and the content difference of 10-DAB in different picking parts,different growth years and different harvesting times was detected by Ultra-high Performance Liquid Chromatography(UPLC).The results showed that the optimum extraction conditions of 10-DAB from T.wallichiana var.mairei were as follows:the solid-liquid ratio was 1∶15(g/mL),the extraction time was 23 min,and the ultrasonic temperature was 40℃.Under these conditions,the content of 10-DAB was 1.18%,and the relative deviation from the theoretical value was 0.42%.The results of UPLC detection showed that the content of 10-DAB in the leaves of T.wallichiana var.mairei was significantly higher than that in branches and barks,and the annual cumulative content of 10-DAB was obviously affected by the season,reaching the highest value in October.This study can provide a reference for the extraction and utilization of 10-DAB in T.wallichiana var.mairei.