大豆优异种质蛋白质和脂肪含量QTL挖掘

QTL Mining of Protein and Oil Content in Elite Soybean Germplasm

大豆是全球重要的豆科作物,是人们食用和饲用主要的蛋白质和油脂来源。大豆蛋白质和脂肪含量受多基因控制且易被环境等因素影响,发掘高蛋白和脂肪基因位点对于定向培育大豆新品种具有重要意义。本研究选用由高产优质品种黑农84与蛋白含量较高品系京河4号杂交构建的880个家系组成的遗传分离群体,利用中豆芯一号SNP标记和SSR分子标记技术鉴定F2群体的基因型,结合蛋白质、脂肪含量表型,通过QTL IciMapping4.2的完备区间作图法(ICIM-ADD)定位获得2个蛋白QTL和2个脂肪QTL。q Pro_11_1和qOil_11_1,位于分子标记SSR_11_1087与SSR_11_1090之间,区间大小为126.27 kb,遗传贡献率分别为4.05%和3.23%,共有注释基因5个。qPro_14_1和qOil_14_1,位于SSR_14_0421与SSR_14_0429之间,区间大小为246.09kb,遗传贡献率分别为4.67%和7.13%,共有注释基因15个。本研究定位的稳定的蛋白质含量位点和脂肪含量新位点,为大豆高蛋白和高油分子标记辅助选择育种和基因图位克隆奠定了基础。

Soybean is an important legume crop in the world,and serves as the main donor of protein and oil for human diet and animal feeding.The protein and oil content are genetically controlled by multiple genes,and are largely environment-dependent.It is of great significance to explore high protein and oil loci in targeted breeding of new soybean varieties.In this study,a genetic segregation population including 880 families was generated by Heinong 84(high yield and quality)crossed with Jinghe 4(high protein content),and subjected for the protein and oil content quantification.The genotyping was conducted using ZDX1 SNP array and SSR molecular markers,combine.Two protein QTL and two oil QTL were identified through the complete interval mapping method(ICIM-ADD)using QTL IciMapping 4.2.qPro_11_1 and qOil_11_1 were co-localized in an interval of 126.27 kb between the molecular markers SSR_11_1087 and SSR_11_1090,showing the genetic contribution rate of 4.05%and 3.23%respectively,as well as five annotation genes in the region.qPro_14_1 and qOil_14_1 were co-localized between the molecular markers SSR_14_0421 and SSR_14_0429,with the interval size of 246.09 kb with 15 annotation genes,showing the genetic contribution rate of 4.67%and 7.13%respectively.Collectively,this study identified the QTL loci on protein content and oil content,thus laying a foundation for the marker-assisted selection breeding and gene map-based cloning of soybean with high protein and high oil content.