广谱抗稻瘟病水稻保持系赣香B的抗性遗传解析

Genetic Dissection of Broad Spectrum Resistance of the Rice Maintainer Ganxiang B

【目的】赣香B是江西省农业科学院水稻研究所育成的水稻野败型保持系,在江西、福建、贵州、湖北和浙江等多地都表现较强的稻瘟病广谱抗性。对赣香B的稻瘟病抗性位点的遗传解析,有助于今后稻瘟病抗性育种工作。【方法】通过抗感株系基因组比较、稻瘟病抗性位点标记检测、抗性位点测序和片段代换系表型鉴定,分析赣香B中的稻瘟病抗性基因位点。【结果】赣香B中含稻瘟病抗性基因Pish、Pia和Pita。在稻瘟病高感株系中导入Pish后,抗性得到显著增强;而导入Pita或者Pia后,抗性略微增强或者没有显著效果。【结论】赣香B携带多个稻瘟病抗性基因,其中,Pish是主效抗性基因,表现出广谱稻瘟病抗性。

【Objective】Ganxiang B is a WA type rice maintainer line developed by the Rice Research Institute of Jiangxi Academy of Agricultural Sciences.It shows broad spectrum resistance against rice blast in Jiangxi,Fujian,Guizhou,Hubei,and Zhejiang Provinces.Genetic analysis of blast resistance loci in Ganxiang B will contribute to the future work for blast resistance breeding.【Method】The rice blast resistance loci in Ganxiang B were analyzed by genome comparison between resistant and susceptible lines,marker detection of rice blast resistance locus,resistance gene sequencing,and phenotypic identification of segment substitution lines.【Results】There were resistance genes Pish,Pia,and Pita in Ganxiang B.After introgressed with Pish,the blast resistance of highly susceptible line was significantly enhanced.However,after introgressed with Pita or Pia,the resistance was slightly enhanced or remained unchanged.【Conclusion】Ganxiang B carries several blast resistance genes.Among them,Pish is the main resistant gene,showing broad resistance to rice blast.