QuEChERS-LC-MS/MS法测定猪肉中44种兽药残留

Determination of 44 Veterinary Drugs Residues in Pork by QuEChERS-LCMS/MS Method

本文建立了高效液相色谱-串联质谱法(LC-MS/MS)同时测定猪肉中44种兽药残留的检测方法。样品中的4种四环素类、16种喹诺酮类、24种磺胺类化合物用1%甲酸乙腈溶液提取后,经QuEChERS净化,氮吹浓缩后通过C18色谱柱分离,并采用基质标准曲线法定量。结果表明44种化合物在2.0~100.0μg/kg范围内线性关系良好,相关系数R均大于0.995,加标回收率在70.8%~106.2%之间,相对标准偏差为1.1%~8.1%(n=6)。该方法操作简便、经济快速、灵敏准确,适用于猪肉中多种兽药残留的快速筛查。

A high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for the simultaneous determination of 44 veterinary drug residues in pork.Four tetracyclines,16 quinolones and 24 sulfonamides in the sample were extracted with 1%formic acid acetonitrile solution and purified by QuEChERS method.The concentrated solution after nitrogen blowing was separated by Cig chromatographic column and quantified by matrix standard curve method.The results showed that the linear range was from 2.0μg/kg to 100.0μg/kg and the correlation coefficient was greater than 0.995.The recoveries were 70.8%~106.2%,and the relative standard deviations(RSD,n=6)were 1.1%~8.1%.The method is simple,economical,rapid,sensitive and accurate,which can be applied to rapid screening of veterinary drug residues inpork.