一种马铃薯脱毒种苗无糖组织培养快繁方法

A Method of Sugar Free Tissue Culture and Rapid Propagation of Virus-free Potato Seedlings

为提高马铃薯优良脱毒种苗质量,优化种苗生产,研究无糖培养在马铃薯脱毒种苗快繁中的应用。以马铃薯早熟品种‘大西洋’的脱毒试管苗为材料,以MS培养基附加4%蔗糖为对照(CK),探究无糖培养过程中不同培养支撑物、接种方式、首次通入CO_(2)时间、CO_(2)通入浓度及光照强度对试管苗生长发育的影响。结果表明,无糖培养25 d后,双节段接种的马铃薯种苗株高、节间数、去根鲜质量显著大于单节段接种的种苗,双节段接种更适合马铃薯脱毒种苗的快繁。马铃薯脱毒苗在以塑料颗粒为支撑物的无糖培养盒中培养长势最好,但价格较昂贵,综合考虑推荐以蛭石为支撑物。接种后3 d通入CO_(2)的马铃薯试管苗株高为10.29 cm,显著高于其他处理;在光照强度8000~8500 lx、CO_(2)浓度1×10^(6)mg/m^(3)无糖培养条件下,马铃薯脱毒苗长势最好,株高10.28 cm、茎粗1.55 cm、鲜质量0.90 g,显著高于其他处理。

In order to improve the quality of virus-free potato seedlings and optimize potato seedlings production,the application of sugar free culture in rapid propagation of virus-free potato seedlings was studied.The virus-free plantlets of potato early maturing variety‘Atlantic'were used as materials.The results showed that after 25 days of sugar free culture,the plant height,internode number,and rootless fresh quality of potato seedlings double stage inoculation were significantly greater than those inoculated in single segment,double stage inoculation was more suitable for rapid propagation of virus-free potato seedlings.The segments of potato virus-free seedlings grew best in a sugar free culture box supported by plastic particles,but the price was relatively expensive.Therefore,it was recommended to use vermiculite as the support for comprehensive consideration.The plant height of potato plantlets injected with CO_(2) 3 days after inoculation was 10.29 cm,significantly higher than that of other treatments.Under the conditions of light intensity of 8000-8500 lx and CO_(2)concentration of 1×10^(6) mg/m^(3) sugar-free culture,potato virus-free seedlings had the best growth potential,with a plant height of 10.28 cm,stem diameter of 1.55 cm,and fresh weight of 0.90 g,significantly higher than other treatments.