安全剂环丙磺酰胺和双苯噁唑酸对玉米苯唑氟草酮药害的解毒作用

The Detoxification Effect of Safeners of Cyclopropanesulfonamide and Isoxadifen-ethyl against Fenpyrazone in Corn

为了优化苯唑氟草酮在玉米田的应用技术。以‘辽单127’玉米(Zea mays Linn.)为测试靶标,采用温室盆栽法研究了安全剂环丙磺酰胺和双苯噁唑酸对玉米体内苯唑氟草酮的解毒效果,并通过对谷胱甘肽转移酶(GSTs)活性的测定初步研究了环丙磺酰胺的解毒机理。结果显示:在苯唑氟草酮有效成分量360 g/hm^(2)茎叶喷雾处理下,7.5、15、30 g/hm^(2)的环丙磺酰胺和双苯噁唑酸均可提高‘辽单127’玉米对苯唑氟草酮的耐药性,株高和鲜重保护率在117%~269%之间。其中,加入环丙磺酰胺15 g/hm^(2)的苯唑氟草酮处理组的株高和鲜重保护率在所有处理组中均达到了最高,株高保护率为133%,鲜重保护率为269%。添加安全剂环丙磺酰胺15 g/hm^(2)后,苯唑氟草酮处理的玉米GSTs比活力在施药后1~9 d内均显著提高,在施药后第5天,GSTs比活力达到最大。结果表明添加安全剂环丙磺酰胺提高了玉米体内GST活性,降低了苯唑氟草酮对玉米生长的抑制作用,减轻了苯唑氟草酮对玉米的药害。

In order to optimize the application technology of fenpyrazone in corn(Zea mays Linn.) fields,the detoxification effect of safeners cyclopropanesulfonamide and isoxadifen-ethyl on fenpyrazone was studied in greenhouse targeted by‘Liaodan 127'maize,and the detoxification mechanism of cyclopropanesulfonamide was preliminarily studied by measuring the activity of glutathione transferase(GSTs) in maize.The results showed that under the treatment of effective component of fenpyrazone 360 g(a.i.)/hm^(2)stem and leaf spray,7.5,15,30 g a.i./hm^(2)of cyclopropanesulfonamide and isoxadifen-ethyl could improve the tolerance of‘Liaodan 127'maize to fenpyrazone,and the protection rate of plant height and fresh weight was between117% and 269%.Among them,the protection rate of plant height and fresh weight of the treatment group treated with fenpyrazone combined with cyclopropanesulfonamide 15 g a.i./hm^(2)reached the highest,with the protection rate of plant height and fresh weight of 133% and 269%,respectively.After adding the cyclopropanesulfonamide at 15 g a.i./hm^(2),the enzymatic specific activity of GSTs in maize treated with fenpyrazone increased significantly within 1-9 days after application,and reached the maximum on the 5th day after application.In conclusion,cyclopropanesulfonamide increased the GSTs activity in maize,reduced the inhibitory effect of fenpyrazone on maize growth,and alleviated the herbicide damage in maize caused by fenpyrazone.