4种食源性多糖的单糖组成及其对耐甲氧西林金黄色葡萄球菌抑制作用的研究

Four Food-derived Polysaccharides:Monosaccharide Composition and Their Inhibitory Effects on Methicillin-resistant Staphylococcus aureus

旨在为致病菌耐药性问题提供了可能的替代方案。以木耳、山药、百合、枸杞为原料,采用水提醇沉法、离子色谱法、结晶紫染色法等技术对比分析4种食源性多糖的单糖组成及其对致病菌耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)的抑制效应。结果表明:4种多糖均以葡萄糖为主要单糖,木耳多糖对MRSA的生长、生物被膜的形成、细胞粘附和毒性均有明显的抑制作用;百合多糖和山药多糖仅对MRSA的细胞粘附和毒性有较为显著的抑制作用,对MRSA的生长和生物被膜的形成无明显的抑制效应;枸杞多糖对MRSA几乎没有抑制能力。木耳多糖对致病菌MRSA的抑制能力优于其他3种食源性多糖,作为潜在抗MRSA的天然产物具有进一步表征及优化的价值。

The research aims to provide a possible alternative to the problem of drug resistance in pathogenic bacteria.With fungus,yam,lily and wolfberry as raw materials,water extraction and alcohol precipitation method,ion chromatography,crystal violet staining and other techniques were used to compare and analyze the monosaccharide composition of four kinds of foodborne polysaccharides and their inhibitory effect on methicillin-resistant Staphylococcus aureus(MRSA).The results showed that glucose was the main monosaccharide of the four polysaccharides.Auricularia polysaccharides had significant inhibitory effects on the growth,biofilm formation,cell adhesion and toxicity of MRSA.Lilium polysaccharide and yam polysaccharide only had significant inhibitory effect on the cell adhesion and toxicity of MRSA,but had no significant inhibitory effect on the growth of MRSA and the formation of biofilm.Lycium barbarum polysaccharide had little inhibitory effect on MRSA.Auricularia auricularia polysaccharide has better inhibition ability against pathogenic bacteria MRSA than the other three foodborne polysaccharides.As a natural product with potential antibacterial applications against MRSA,it has the value of further characterization and optimization.