妊娠晚期孕妇阴道微生态现状分析

Analysis of Vaginal Microecology in the Late Trimester of Pregnancy

目的分析妊娠晚期孕妇阴道微生态现状。方法随机收集2021年7~8月山西省儿童医院(山西省妇幼保健院)收治的60例妊娠晚期孕妇阴道分泌物样本,使用NovaSeq 6000平台对16S rDNA基因的V3~V4区域进行测序,分析其阴道微生态现状。结果阴道微生态正常组(AN组)以乳酸杆菌为主;阴道微生态失调组(BU组)以巨型球菌属、加德纳菌属、普雷沃菌属、小杆菌属、纤毛菌属、瘤胃球菌属和阿托波菌属为主,多样性高于AN组。采用PCoA、MRPP和ANOSIM分析Beta多样性,结果表明,AN组孕妇阴道微生物菌群与BU组间比较差异有统计学意义(P<0.05)。结论妊娠晚期孕妇阴道微生态失调主要表现为乳酸杆菌缺乏和高细菌多样性。

Objective To analyze the current situation of vaginal microecology in the late trimester of pregnancy.Methods Sixty vaginal secretion samples were randomly collected from women in the late trimester of pregnancy admitted to Shanxi Children's Hospital(Shanxi Maternal and Child Health Hospital)in July to August 2021,and the V3-V4 region of the 16S rDNA gene was sequenced using the NovaSeq 6000 platform to analyze their vaginal microecology status.Results Lactobacillus was dominant in normal vaginal microecology group(AN group);the disorder vaginal microecology group(BU group)was mainly composed of megasphaera,gardnerella,prevotella,dialister,sneathia,shuttleworthia and atopobium,and the diversity was higher than that of AN group.PCoA,MRPP and ANOSIM were used for analysis Beta diversity,the results showed that there was significant difference in vaginal microflora between AN group and BU group(P<0.05).Conclusion The vaginal microecology disorder in the late trimester of pregnancy is mainly manifested as lactobacillus deficiency and highbacterial diversity.