高效液相色谱法同时测定植物油中3种维生素A和9种维生素E

Simultaneous determination of 3 kinds of vitamin A and 9 kinds of vitamin E in vegetable oil by high performance liquid chromatography

建立1种通过高效液相色谱法同时测定植物油中3种维生素A(维生素A-全反式酯、维生素A-醋酸酯、维生素A-棕榈酸酯)和9种维生素E(δ-生育三稀酚、β-生育三稀酚、γ-生育三稀酚、α-生育三稀酚、δ-生育酚、β-生育酚、γ-生育酚、α-生育酚、d-α-醋酸酯)的方法,并对13种植物油进行检测。结果表明:方法的色谱柱为CAPCELL PAK PFP(150 mm×4.6 mm,5μm)、提取剂为乙醇、2,6-二叔丁基对甲酚(BHT)添加量为0.01g、流动相为乙醇-水,梯度洗脱。建立的方法在质量浓度0.5~20μg/mL范围内,线性关系良好(R^(2)>0.999),方法的检出限为0.100~2.80 mg/kg、定量限为0.332~13.32 mg/kg、相对标准偏差(RSD)≤5.27%、回收率为80.67%~98.42%。建立的方法可用于植物油中3种维生素A和9种维生素E的同时检测。

A method was established for simultaneous determination of 3 kinds of vitamin A(vitamin Aall-trans ester、vitamin A-acetate、vitamin A-palmitate)and 9 kinds of vitamin E(δ-Tocotrienol、β-Tocotrienol、γ-Tocotrienol、α-Tocotrienol、δ-Tocopherol、β-Tocopherol、γ-Tocopherol、α-Tocopherol、d-α-Tocopherol acetate)in vegetable oil by high performance liquid chromatography,and 13 kinds of vegetable oil were detected.The results showed that the chromatographic column was CAPCELL PAK PFP(150 mm×4.6 mm,5μm),ethanol was used as the extractant,0.01 g 2,6-di-tert-butyl-p-cresol(BHT)was added,and the mobile phase was ethanol-water with gradient elution.The method had a good linear relationship in the mass concentration range of 0.5-20μg/mL(R^(2)>0.999).The detection limit was 0.100-2.80 mg/kg,and the limit of quantitation was 0.332-13.32 mg/kg.The relative standard deviation was not more than 5.27%,and the recovery rate was 80.67%-98.42%.The established method can be used for the simultaneous determination of 3 vitamin A and 9 vitamin E in vegetable oil.