红景天内生真菌Fusarium sp.HJT-P-2次级代谢产物及其促进植物生长活性的研究

Study on the secondary metabolites of rhodiola endophytic fungus Fusarium sp.HJT-P-2 in and its growth-promoting activity

目的研究长白红景天植物内生真菌Fusarium sp.HJT-P-2次级代谢产物,以期得到结构新颖的先导化合物。方法采用硅胶柱色谱和高效液相色谱等方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构,并对部分化合物进行模式植物拟南芥种子植物生长影响活性研究。结果内生真菌Fusarium sp.HJT-P-2次级代谢产物中分离得到6个化合物:1个新化合物1和5个萘醌类化合物2、3、4、5、6。化合物2、3对拟南芥种子根的生长抑制作用相对较弱,化合物5、6对拟南芥种子根的生长抑制作用相对较强,化合物4在质量浓度为2 mg·L^(-1)时,相对于其前后浓度对根生长的抑制现象出现反常行为促进了根的生长。化合物6在质量浓度为20 mg·L^(-1)时,所有种子均未发芽,浓度过高时出现致死现象。结论为发现新型活性先导化合物提供研究思路,同时为绿色植物激素的开发提供一定的物质基础。

Objective To study the secondary metabolites of the endophytic fungus Fusarium sp.HJT-P-2 of Rhodiola serrata,in order to obtain active compounds with novel structures.Methods Silica gel column chromatography and high performance liquid chromatography were used for separation and purification.The structures of the compound were identified through physical and chemical properties and spectral data analysis,and the activity of some compounds was studied on the seed plant growth of model plant Arabidopsis thaliana.Results Six compounds were isolated from the secondary metabolites of the endophytic fungus Fusarium sp.HJT-P-2,one new compound 1 and five naphthoquinone compounds 2,3,4,5 and 6.Compounds 2 and 3 had relatively weak inhibitory effects on Arabidopsis seed roots.Compounds 5 and 6 had relatively strong inhibitory effects on Arabidopsis seed roots.When the concentration of compound 4 was 2 mg·L^(-1),the inhibitory effect was relatively weak,on the other hand,it could promote the growth of roots.When the concentration of compound 6 was 20 mg·L^(-1),all seeds didn′t germinate,and can lead to the phenomenon of plant death when high concentration of 6 was used.Conclusion This study provides research ideas for the discovery of new active lead compounds,and provides a certain material basis for the development of green plant hormones.