芪术颗粒挥发油β-环糊精包合物的制备及表征

Preparation ofβ-cyclodextrin inclusion complexes of volatile oil in Qizhu granules and its characterization

目的:制备芪术颗粒中挥发油β-环糊精包合物。方法:以包合率为指标进行单因素实验,进行包合时间、包合温度和挥发油与β-环糊精比例的工艺参数优选;以包合率、包合物得率作为指标,采用Box-Behnken响应面法对包合物制备工艺进行优化。采用薄层色谱、红外光谱、差示扫描量热法对所得包合物进行表征。结果:最佳工艺为:挥发油与β-环糊精比例1∶9,包合温度64℃,包合时间1 h,所得包合物的包合率为90.68%,包合物得率为80.40%。表征结果均证明包合物形成。结论:该工艺稳定可靠,可用于制备芪术颗粒中挥发油β-环糊精包合物。

Objective:To prepare theβ-cyclodextrin inclusion complexes of volatile oil in Qizhu granules.Methods:The technology parameters like inclusion time,inclusion temperature,and the ratio of volatile oil toβ-cyclodextrin in the preparation process of inclusion complexes were investigated by single factor experiment with inclusion rate as the index.With inclusion rate and inclusion yield as indexes,Box-Behnken response surface method was used to optimize the preparation process of inclusion complexes.Thin-layer chromatography,infrared spectroscopy and differential scanning calorimetry were utilized to characterize the inclusion complexes.Results:The optimal conditions were as follows:9∶1 for volatile oils-(β-cyclodextrin)ratio,64℃for inclusion temperature,and 1 h for inclusion time.The inclusion rate and yield of the obtained inclusion complexes were 90.68%and 80.40%,respectively.All characterization results revealed the formation of inclusion complexes.Conclusion:The established method is stable and feasible,which can be used for preparing theβ-cyclodextrin inclusion complexes of volatile oil in Qizhu granules.