五味子甲素对炎性肠病小鼠结肠NF-κB/COX-2信号通路的影响

Influence of Deoxyschizandrin on NF-κB/COX-2 Signal Pathway in Colon of Mice with Inflammatory Bowel Disease

目的:考察五味子甲素对三硝基苯磺酸所致炎性肠病小鼠核转录因子κB(nuclear factor-κB,NF-κB)/环氧合酶2(cyclooxygenase,COX-2)通路的抑制作用。方法:采用三硝基苯磺酸灌肠诱导小鼠炎性肠病,采用ELISA、免疫组化、WB法测定小鼠体内NF-κB及COX-2表达,采用RT-PCR法测定小鼠结肠组织NF-κB及COX-2 mRNA表达,观察小鼠体质量、腹泻症状和疾病活动指数。结果:炎性肠病小鼠体内NF-κB、COX-2含量、蛋白及其mRNA表达增多,五味子甲素可抑制NF-κB、COX-2 mRNA和蛋白表达,抑制炎性肠病小鼠体质量减轻和疾病活动指数,改善小鼠腹泻情况。结论:五味子甲素可通过抑制NF-κB/COX-2通路的激活而抑制炎症反应,对三硝基苯磺酸诱导的炎性肠病小鼠具有治疗作用。

Objective:To survey the inhibitory effects of deoxyschizandrin on NF-κB/COX-2 signal pathway in mice suffering TNBS-induced inflammatory bowel disease(IBD).Methods:TNBS enema was adopted to induce IBD in mice,ELISA,immunohistochemical method and WB method were applied to detect the expressions of NF-κB and COX-2 in mice,RT-PCR method was utilized to measure the expression of NF-κB and COX-2 mRNA,to observe body weight,the symptoms of diarrhea and disease activity index(DAI).Results:The expressions of NF-κB and COX-2 mRNA and protein increased in IBD mice,while deoxyschizandrin could inhibit the expressions of NF-κB and COX-2 mRNA and protein,restrain the reduction of body weight and DAI in IBD mice and improve diarrhea.Conclusion:Deoxyschizandrin could inhibit inflammatory reaction through the inhibition of the activation of NF-κB/COX-2 signal pathway,thus showing therapeutic effects on TNBS-induced IBD in mice.