LED光质对乌饭树叶片叶绿素荧光参数的影响

Effect of different LED light quality on chlorophyllfluorescence parameters of Vaccinium bracteatum leaves

为探讨LED光质对乌饭树叶片叶绿素荧光参数的影响,以乌饭树幼苗为试验材料,采用4种LED单色光质红光(R)、黄光(Y)、蓝光(B)和紫外光(UV)培养幼苗30 d,以白光(W)为对照,研究LED不同光质对乌饭树叶片光合色素和叶绿素荧光参数的影响。结果显示:相比白光对照(W),紫外光(UV)和蓝光(B)能够显著提高叶片的叶绿素a和叶绿素b的含量(P<0.05);黄光(Y)会显著降低叶绿素a的含量(P<0.05),LED单色光质都能显著提高类胡萝卜素的含量(P<0.05)。LED单色光质处理均显著提高了叶PSⅡ潜在光化学活性(F_(v)/F_(o))和PSⅡ最大光化学效率(F_(v)/F_(m))(P<0.05),紫外光(UV)处理的叶片F_(v)/F_(m)值最高,蓝光(B)其次;紫外光(UV)和蓝光(B)处理下叶片的PSⅡ有效光化学量子产量(F′v/F′m)和PSⅡ实际光化学效率(ΦPSⅡ)显著提高(P<0.05);黄光(Y)处理下叶片的光化学猝灭(q P)显著降低(P<0.05),而不同光质处理对乌饭树叶片的非光学猝灭(NPQ)无明显影响。结果表明,紫外光(UV)和蓝光(B)处理下叶片光合色素含量显著增加,植物叶片对光能的利用效率更高,叶片较少产生热耗散,试验结果可以为今后乌饭树工厂化生产时光质选择提供依据。

In order to investigate the effect of LED light quality on chlorophyll fluorescence parameters in Vaccinium bracteatum leaves,using V.bracteatum seedlings as experimental materials,seedlings were cultured under four LED monochromatic light qualities:red light(R),yellow light(Y),blue light(B),and ultraviolet light(UV)for 30 days,compared with white light(W)to study the effects of different LED lights on photosynthetic pigments and photosynthetic mechanisms in V.bracteatum leaves.The results showed that compared with white light(W),ultraviolet light(UV)and blue light(B)could significantly increase the contents of chlorophyll a and chlorophyll b in leaves(P<0.05);yellow light(Y)could significantly reduce the content of chlorophyll a(P<0.05),and LED monochromatic lights could significantly increase the content of carotenoid(P<0.05).LED monochromatic light treatments significantly improved the potential photochemical activity(F_(v)/F_(o))and maximum photochemical efficiency(F_(v)/F_(m))of PSⅡ(P<0.05),while UV treatment had the highest F_(v)/F_(m)value,followed by blue light(B).Under ultraviolet(UV)and blue(B)light treatments,the effective photochemical quantum yield of PSⅡ(F′v/F′m)and the actual photochemical efficiency of PSⅡ(ΦPSⅡ)in leaves were significantly increased(P<0.05);The photochemical quenching(q P)of leaves under yellow light(Y)treatment significantly decreased(P<0.05),while different light quality treatments had no significant impact on NPQ.The results showed that ultraviolet(UV)and blue(B)light treatments could increase photosynthetic pigment content of leaves significantly,lead to higher utilization efficiency of light energy in plant leaves and less heat dissipation in leaves.The results of this experiment can improve the selection of light quality for future industrial production of V.bracteatum.