摘要
In this study,different molecular weight(MW)carboxymethyl chitosans(CM-chitosan)nanoparticles were prepared by ionic gelification.The particle size of nanoparticles was around 180–250 nm by dynamic light scattering(DLS)and transmission electron microscope(TEM).With the increase of CM-chitosan nanoparticles concentration from 2 to 200μg/mL,the growth inhibition effects on the keloid fibroblast increased.At the concentration of 100μg/mL,CM-chitosan nanoparticles withMW6.3 kDa had a significant inhibitory effect(inhibition ratio 48.79%)of the proliferation of keloid fibroblast.Compared with CM-chitosan solution,the inhibition of CM-chitosan nanoparticles were lower in prior period and similar in later period.By analyzing the different effects of chitosan,CM-chitosan solution and CM-chitosan nanoparticles on proliferation of keloid fibroblast,we have found that the carboxylmethyl groups of CM-chitosan play an important role in inhibition of proliferation of keloid fibroblast.
基金
This work was supported by the National Natural Science Foundation of China(Grant No.81071274).
