微RNA-221、微RNA-182-5p及肿瘤坏死因子-α对肺炎支原体感染患儿伴气道高反应的预测价值

Predictive value of microRNA-221,miR-182-5p and TNF-αin children with Mycoplasma pneumoniae infection with airway hyperresponsiveness

目的探讨微RNA(miR)-221、miR-182-5p及肿瘤坏死因子-α(TNF-α)对肺炎支原体(MP)感染患儿气道高反应性(AHR)的预测价值。方法选取2020年1月至2022年12月我院儿科收治的328例MP感染患儿为研究对象,并按患儿是否伴发AHR分成AHR组(n=118)和非AHR组(n=210)。检测两组的miR-221、miR-182-5p及TNF-α水平,并获取相关临床资料,分析MP感染患儿发生AHR的危险因素,受试者工作特征曲线(ROC)评估miR-221、miR-182-5p及TNF-α对MP感染患儿发生AHR的预测价值。结果Logistic分析显示,病程、家族哮喘史、FEV1、FVC、miR-221、miR-182-5p及TNF-α水平均是MP感染患儿发生AHR的独立危险因素(P<0.05)。ROC曲线显示,miR-221、miR-182-5p及TNF-α水平单独及联合预测MP感染患儿发生AHR的AUC分别为0.756、0.861、0.618、0.899,三者联合预测MP感染患儿发生AHR的AUC高于单独预测(P<0.05)。结论miR-221、miR-182-5p及TNF-α是MP感染患儿发生AHR的独立危险因素,miR-221、miR-182-5p及TNF-α水平对MP感染患儿发生AHR均具有一定预测价值,三者联合预测时价值更高。

Objective To investigate the predictive value of microRNA-221(miR-221),miR-182-5p and tumor necrosis factor-α(TNF-α)on airway hyperresponsiveness(AHR)in children with Mycoplasma pneumoniae(MP)infection.Methods 328 children with MP infection admitted to our hospital from January 2020 to December 2022 were selected as the study object,and the children were divided into AHR group(n=118)and non-AHR group(n=210)according to whether they had AHR with or without AHR.To detect miR-221,miR-182-5p and TNF-αlevels in both groups and obtain relevant clinical information to analyze the risk factors for the development of AHR in children with MP infection.and the subject operating characteristic curve(ROC)was used to assess the predictive value of miR-221,miR-182-5p and TNF-αfor the development of AHR in children with MP infection.Results Logistic analysis showed,family history of asthma,FEV1,FVC,miR-221,miR-182-5p and TNF-αlevels were all independent risk factors for the development of AHR in MP-infected children(P<0.05).The ROC curves showed that the AUC of miR-221,miR-182-5p and TNF-αlevels alone and in combination predicted the occurrence of AHR in MP-infected children was 0.756,0.861,0.618 and 0.899,respectively,and the AUC of MP infection was higher than that of MP infection alone(P<0.05).Conclusions miR-221,miR-182-5p and TNF-αare independent risk factors for the development of AHR in MP-infected children.miR-221,miR-182-5p and TNF-αlevels have some predictive value for the development of AHR in MP-infected children,and the value is higher when the three are combined.