丝素蛋白茜草素复合纤维膜的制备及应用

Preparation and application of silk fibroin alizarin composite fiber membranes

为提高丝素蛋白纤维膜的止血抗菌性能,以茜草素为止血抗菌剂,六氟异丙醇为溶剂,采用静电纺丝法制备丝素蛋白茜草素复合纤维膜,对不同质量比的丝素蛋白茜草素复合纤维膜形貌、吸水率、止血性能和抗菌性能进行了测试分析。结果表明:不同质量比的丝素蛋白茜草素复合纤维膜呈光滑致密、无串珠的网状结构,并具有较高的吸水率;随着茜草素含量的增加,复合纤维膜的抗菌性呈显著增加趋势,当丝素蛋白茜草素质量比为10∶1时,丝素蛋白茜草素复合纤维膜对大肠杆菌和金黄色葡萄球菌的抑菌率分别达到(92.02±1.03)%和(98.03±0.28)%,其止血性能明显优于市售止血纱布。

Sudden bleeding accident is one of the important causes of human death,and the death caused by massive bleeding is a considerable medical problem.Therefore,the rapid control of bleeding has become the focus of clinical treatment,and is of important significance in military trauma and traffic accidents.Currently,few antibacterial hemostatic materials developed can simultaneously meet the requirements of low price,rapid hemostatic and low side effects,so there is still huge potential in the development of safe and non-toxic antibacterial hemostatic materials.Silk fibroin is a natural polymer,and has been studied and applied in the field of biomedical materials due to its good hemostatic performance,biocompatibility and biodegradability.However,the antibacterial property of the homogenous silk fibroin protein material is insufficient,which limits its application in wound dressing.In recent years,although inorganic antibacterial agents and organic antibacterial agents are widely used and have high bacteriostatic efficiency,it is still difficult to avoid the environmental problems caused by chemical synthesis and the drawbacks of harming human health.Natural antibacterial agents have become a better choice in the field of medical materials.Alizarin extracted from the medicinal plant madder,is a natural antibacterial agent,has few side effects on the human body,and has hemostatic,anti-inflammatory and other effects.In this paper,alizarin was used as natural antiseptic and hexafluoroisopropyl alcohol was used as solvent to dissolve silk fibroin protein and prepare a spinning solution.The microscopic morphology,water absorption,hemostatic and antibacterial properties of silk fibroin alizarin composite fiber membranes were investigated under different proportions.The results show that,compared with pure silk fibroin fiber membranes,the diameter distribution of the composite fiber membrane with alizarin has no obvious difference.The fibers in the membrane are compact and smooth without beading morphology.At the same time,new absorption peaks appear on the infrared curve of the composite fiber membrane,corresponding to the out-of-plane bending vibration of the C—H bond in the benzene ring,the in-plane bending vibration of C—H plane,and the out-of-plane bending vibration of C—H.The changes of the above peaks indicate the existence of alizarin in the silk fibroin film.Compared with hemostatic gauze,the water absorption of silk fibroin film has a significant advantage,and the water absorption of the composite film gradually decreases with the increase of alizarin content.Compared with the BCI value of hemostatic gauze,the BCI value of the composite film decreases gradually with the increase of alizarin content,and the addition of alizarin significantly improves the hemostatic performance of the film.Compared with pure silk fibroin membrane,the antibacterial activity of the composite membrane is significantly improved,and the antibacterial rate increases with the increase of the ratio of silk fibroin to alizarin.At the same time,under the condition that the mass ratio of silk fibroin to alizarin of 10:1,the antibacterial rates of the composite membrane are both more than 90%against and,showing strong antibacterial effect.Silk fibroin and alizarin are natural substances,with little harm to human body.Silk alizarin composite membranes have good effect in hemostasis and antibacterial test,and show great application prospect in the development of silk fibroin wounds.