基于转录组测序揭示光质对刺五加实生苗基因表达的调控作用

Sequencing and analysis of transcriptome to reveal regulation of gene expression in Acanthopanax senticosus seedling under light quality

目的对刺五加Acanthopanax senticosus实生苗进行转录组测序,分析其响应不同光质调控的分子机制。方法以生长60 d的刺五加实生苗为材料,设置不同光质处理组(LED-1、LED-2)和对照组[高压钠灯(high pressure sodium,HPS)],应用Illumina HiSeqTM对其叶片进行转录组测序,对测序后的结果进行基因功能注释、差异表达基因(differentially expressed genes,DEGs)筛选和共表达网络分析。结果转录组测序共获得126252条Unigene。其中92681条被注释,3个处理组中筛选出DEGs7664个。基因本体(gene ontology,GO)富集结果表明,DEGs在代谢过程、刺激响应、细胞结构、催化活性等功能中得到显著富集。京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)途径富集分析表明,DEGs显著富集在植物激素传导、糖代谢、苯丙烷类生物合成、类黄酮生物合成。适度光质胁迫可以促进刺五加实生苗中苯丙素类化合物生物合成途径关键酶基因的表达,是促进刺五加有效成分累积的基础。结论通过高通量转录组测序,揭示了不同光质对刺五加实生苗基因表达的调控特征,可为深入研究刺五加药效成分的生物合成机制及栽培中的光环境设置提供科学依据。

Objective To analyze the molecular mechanism of different tissues of Ciwujia(Acanthopanax senticosus)seedling in response to light quality by transcriptome sequencing.Methods The seedlings of 60 d A.senticosus from moderate light quality group and control group(LED-1,LED-2)were used as the test material.And the transcriptome sequencing analysis was carried out by using Illumina HiSeqTM.After obtaining transcriptome data,gene function annotation,differentially expressed genes(DEGs)screening and and co-expression network analysis were performed.Results A total of 126252 unigenes were obtained by transcriptome sequencing.Among them,92681 unigenes were annotated,and 7664 DEGs were screened out from three treatment groups.The GO enrichment results showed that the DEGs of the roots and leaves were both significantly enriched in metabolic process,stimulus response,cell structure,catalytic activity and other functions.The KEGG pathway enrichment analysis showed that DEGs in leaves were mainly concentrated in phytohormone transduction,sugar metabolism,phenylpropanoid biosynthesis,and flavonoid biosynthesis.Moderate light quality stress can promote the expression of key enzyme genes of phenylpropanoid biosynthesis pathway in A.senticosus seedlings,which is the basis for promoting the accumulation of effective components of A.senticosus.Conclusion The high-throughput transcriptome sequencing revealed the regulatory characteristics of light quality stress on gene expression in different tissues of A.senticosus,which could provide scientific basis for further research on the biosynthesis mechanism of medicinal components of A.senticosus and reasonable luminous environment in cultivation.