地桃花的本草考证、生药学研究及其有效成分定量测定

Herbal textual research,original plant survey and pharmacognostical study of Urena lobata

目的对地桃花Urenalobata进行本草考证、生药学分析及有效成分测定。方法通过查阅文献厘清地桃花的历史使用沿革及植物分布情况,野外实地调研地桃花的植物基原,采集并制作腊叶标本,通过原植物形态、药材性状、显微特征等方法进行生药学研究;采用Waters e2695型液相色谱仪,CAPCELL PAK MGⅡ C_(18)色谱柱(250 mm×4.6 mm,5μm),乙腈-0.1%甲酸溶液为流动相,体积流量1.0 mL/min,梯度洗脱,柱温30℃,PDA检测器,检测波长315 nm,建立地桃花药材中N-反式-阿魏酰酪胺和银锻苷的HPLC测定方法。结果对地桃花药材的性状及显微特征进行了归纳,建立的测定方法稳定性和重复性良好,N-反式-阿魏酰酪胺和银锻苷线性范围0.999~79.890、1.068~85.420μg/mL,R^(2)>0.999,平均回收率95.3%、97.1%。10批样品N-反式-阿魏酰酪胺、银锻苷质量分数为0.003%~0.045%、0.016%~0.046%。结论建立的方法能够有效鉴别地桃花药材,为完善地桃花药材质量标准和地桃花药材收购提供依据。

Objective To carry out the herbal textual research,pharmacognostic analysis and determination of active ingredients of of Ditaohua(Urena lobata L.).Methods The historical use and plant distribution of U.lobata were investigated by referring to the herbal literature.The original plants of U.lobata were surveyed and the original plant specimens were collected through field investigation.The pharmacognosy of U.lobata was studied by morphologic characteristics,character identification,microscopic identifications and TLC identification.A HPLC-UV method for simultaneous determination N-trans-feruloyl tyramine and tiliroside in U.lobata was established by using CAPCELL PAK MGⅡ C_(18) column(250 mm×4.6 mm,5μm),acetonitrile-0.1% formic acid water as mobile phase,gradient elution with the flow rate of 1.0 mL/min,the column temperature was 30℃,the detection wavelength of PDA detector was 315 nm.Result The morphological features and microscopic features of U.lobata were summarized,and the established HPLC method had good stability and repeatability,with wide linear range(0.999-79.890,1.068-85.420μg/mL)and good linearity(R^(2)>0.999).The average recovery was 95.3%,97.1%.The mass fractions of N-trans ferulic tyramine and silver glycoside in 10 batches of samples were 0.003%-0.045% and 0.016%-0.046%.Conclution The established method can effectively identify the U.lobata and provide a theoretical basis for improving the quality standard and acquisition of U.lobata.