基于SLAF-seq对云南常见茄子种质资源遗传多样性分析

Genetic Diversity Analysis of Common Eggplant Germplasm Resources in Yunnan Based on SLAF-seq

以从云南各州市收集的35份茄子种质资源为试材,运用简化基因组测序技术(specific-locus amplified fragment sequencing,SLAF-seq)对其进行遗传多样性分析,研究云南常见茄子种质资源的遗传多样性及开发特异性SNP标记,以期为云南茄子资源种质创新、杂交育种及砧木筛选提供参考依据。结果表明:从35份茄子种质资源中共获得147.42 Mb读长数据,不同资源的reads数目范围在1474699~8628640。测序获得的GC含量在38.29%~46.40%,平均GC含量为39.72%,对照为41.96%。测序质量值Q30在88.11%~93.56%,平均Q30值为91.00%,对照Q30为88.11%。根据测序结果共获得SLAF标签159847个,其中多态性标签共84119个,共筛选出3006351个高效性SNP标记。运用获得的SNP标记对35份茄子资源进行遗传多样性分析、聚类分析及群体PCA分析,结果表明35份茄子种质资源可分为5组。

Thirty five eggplant germplasm resources collected by the research team from Yunnan Provinces and cities were used to analyze their genetic diversity by using specific location amplified fragment sequencing(SLAF-seq).The genetic diversity of common eggplant germplasm resources in Yunnan and develop specific SNP markers were studied,in order to provide reference for germplasm innovation,cross breeding and rootstock selection of eggplant resources in Yunnan.The results showed that 147.42 Mb read length data were obtained from 35 eggplant germplasm resources,and the number of reads from different resources ranged from 1474699 to 8628640.The GC content obtained by sequencing was 38.29%-46.40%,the average GC content was 39.72%,and the control was 41.96%.The sequencing quality value Q30 was in the range of 88.11%-93.56%,the average Q30 value was 91.00%,and the control Q30 was 88.11%.According to the sequencing results,159847 SLAF tags were obtained,included 84119 polymorphic tags,and 3006351 highly efficient SNP tags were screened.The genetic diversity,cluster analysis and population PCA analysis of 35 eggplant germplasm resources were conducted by using the obtained SNP markers.The results showed that 35 eggplant germplasm resources could be divided into 5 groups.