2型糖尿病患者血浆miR-146a和miR-223的表达研究

The expression of miR-146a and miR-223 in plasma of patients with type 2 diabetes

目的探讨2型糖尿病(T2DM)患者血浆miR-146a和miR-223的表达水平,并探讨其对T2DM的临床诊断价值。方法选取2020年6月至2021年6月该院住院的T2DM患者126例作为T2DM组,另随机选取在该院体检中心同期进行健康体检者60例作为健康对照组。收集2组受试者外周血,采用实时荧光定量聚合酶链反应检测血浆miR-146a和miR-223的表达,采用Pearson法分析miR-146a和miR-223与患者监测指标之间的关系,采用受试者操作特征曲线(ROC曲线)评价其对T2DM的诊断价值。结果与健康对照组相比,T2DM组患者血浆miR-146a的表达量升高,miR-223的表达量降低,差异有统计学意义(P<0.01)。相关性分析显示,血浆miR-146a和miR-223的表达存在一定的相关性(r=0.445,P<0.05),同时miR-146a与病程、三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和空腹血糖(FPG)呈正相关(P<0.05);miR-223与病程呈正相关(P<0.05),与TG、TC、LDL-C和FPG呈负相关(P<0.05),两者与体重指数、高密度脂蛋白胆固醇和糖化血红蛋白均无相关性(P>0.05)。ROC曲线分析显示,血浆miR-146a和miR-223诊断T2DM曲线下面积分别为0.792和0.832,联合检测的曲线下面积为0.917。结论血浆miR-146a和miR-223在T2DM患者中存在差异表达,可以作为诊断T2DM潜在的生物学标志物。

Objective To investigate the expression levels of miR-146a and miR-223 in plasma of pa-tients with type 2 diabetes mellitus(T2DM),and to explore their clinical diagnostic value for T2DM.Methods A total of 126 T2DM patients hospitalized in the hospital from June 2020 to June 2021 were selected as the T2DM group,and 60 healthy subjects who underwent physical examination in the physical examination center of the hospital during the same period were randomly selected as the healthy control group.The periph-eral blood of the two groups was collected,and the expression of plasma miR-146a and miR-223 was detected by real-time fluorescence quantitative polymerase chain reaction.Pearson method was used to analyze the rela-tionship between miR-146a and miR-223 and the monitoring indexes of patients.The receiver operating char-acteristic curve(ROC curve)was used to evaluate its diagnostic value for T2DM.Results Compared with the healthy control group,the expression of miR-146a in the plasma of patients with T2DM increased,and the ex-pression of miR-223 decreased(P<0.01).Correlation analysis showed that the expression of miR-146a and miR-223 in plasma had a certain correlation(r=0.445,P<0.05),and miR-146a was positively correlated with the course of disease,triacylglycerol(TG),total cholesterin(TC),low density lipoprotein cholesterol(LDL-C)and fasting plasma glucose(FPG)(P<0.05).MiR-223 was positively correlated with the course of the dis-ease,and negatively correlated with TG,TC,LDL-C and FPG(P<0.05),but not with body mass index,high density lipoprotein cholesterol and glycosylated hemoglobin(P>0.05).ROC curve analysis showed that the area under the curve of plasma miR-146a and miR-223 in diagnosing T2DM was 0.792 and 0.832,respective-ly,and the area under the curve of combined detection was 0.917.Conclusion Plasma miR-146a and miR-223 are differentially expressed in patients with T2DM,which can be used as potential biomarkers for the diagnosis of T2DM.