带状疱疹后神经痛病人Treg/Th17与发病时间的相关性研究

Correlation between Treg/Th17 and time of onset in patients with postherpetic neuralgia

目的:探讨带状疱疹后神经痛(postherpetic neuralgia,PHN)病人外周血中的调节性T淋巴细胞(regulatory Tcells,Treg)与辅助性T淋巴细胞17(helper Tcell,Th17)比值,即Treg/Th17与发病时间的相关性。方法:选取2021年2月至2022年9月青岛市市立医院疼痛科PHN病人48例及同期的22名健康对照者为研究对象,采集他们的各淋巴细胞亚群数据并进行比较。使用流式细胞术检测PHN病人和健康对照者外周血中的Treg细胞和Th17细胞水平,计算Th17与Treg细胞的比率。将PHN病人与健康对照者的Treg细胞比例、Th17细胞比例、Treg/Th17进行对比。统计病人自发病到就诊时间,分析PHN病人Treg/Th17与发病时间的相关性。结果:与健康对照组相比,PHN组外周血中CD4^(+)淋巴细胞数量显著下降,Treg细胞比例,Th17细胞比例,Treg/Th17、CD8^(+)/CD4^(+)比率显著升高;Pearson相关性分析结果显示,PHN病人的Treg/Th17与发病时间呈正相关。结论:带状疱疹病人外周血中Treg细胞与Th17细胞均显著增高,提示两种细胞通过改变免疫系统功能在疾病的转归过程发挥重要作用。PHN病人Treg/Th17与发病时间呈正相关,可能意味着随Treg细胞的相对增多,引起免疫系统功能减退,延长病人病程。

Objective:To investigate the correlation between regulatory T cells(Treg)to helper T cells 17(Th17)ratio(Treg/Th17)in peripheral blood and time of onset in patients with postherpetic neuralgia.Methods:Forty-eight patients with postherpetic neuralgia and 22 healthy controls during the same period were enrolled for the study,and data on each of their lymphocyte subpopulations were collected and compared.The levels of Treg cells and Th17 cells in the peripheral blood of patients with postherpetic neuralgia and healthy controls were measured using flow cytometry,and the ratio of Th17 to Treg cells was calculated.The ratio of Treg cells,the ratio of Th17 cells,and Treg/Th17 were compared between patients and the healthy controls.Patients'time from onset to consultation was counted,and the correlation between Treg/Th17 and onset time in patients with postherpetic neuralgia was analyzed.Results:Compared with the healthy control group,the+number of CD4^(+) lymphocytes in peripheral blood in the patient group decreased significantly and the per-centage of Treg cells,Th17 cells,Treg/Th17,and CD8^(+)/CD4^(+)ratios increased significantly.Pearson cor-relation analysis showed that Treg/Th17 in patients with postherpetic neuralgia was positively correlated with time of onset.Conclusion:Treg and Th17 cells were significantly increased in the peripheral blood of patients with herpes zoster,suggesting that both cells play an important role in the disease by altering the function of the immune system.The positive correlation between Treg/Th17 and time of onset in patients may imply that the increase of Treg cells causes immune system dysfunction and prolongs the course of the disease in patients.